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The 20 kDa myosin light chain of 32P-labeled rat uterus exhibited four spots on two-dimensional gel electrophoretograms; the corresponding autoradiograms revealed that three spots were radioactive. Completely dephosphorylated light chain exhibited three spots on electrophoretograms. Serine and threonine residues of the light chain were found to be(More)
The phosphorylatable myosin light chain of rat uterus was resolved into four spots by two-dimensional gel electrophoresis. Antibody against the 20 kDa light chain from smooth muscle myosin recognized these four spots. Purified light chain exhibited four spots on a diagonal upon isoelectrofocusing in both first and second dimensions, proving that these spots(More)
1. The phosphorylatable light chain from uterine and arterial smooth muscles appear as four spots on two-dimensional gel electrophoretograms due to the existence of isoforms which may be non-, mono- or diphosphorylated. 2. The phosphorylation sites are serine and threonine residues; the phosphoserine to phosphothreonine ratio is smaller, and the extent of(More)
In the present study the effect of phosphorylation of skeletal muscle myosin light chains on the interaction between myosin and actin has been investigated. The actomyosin ATPase activities were determined for synthetic actomyosins formed from either phosphorylated or non-phosphorylated myosin and pure actin, with the help of the luciferin-luciferase(More)
A light chain of 18 000 daltons of native actomyosin isolated from rabbit skeletal muscle was removed by DTNB-treatment. Investigated were the differences in superprecipitation and ATPase activity of LC2-deficient and control actomyosin. The superprecipitation of control actomyosin develops in two phases, with high amplitude and kinetics dependent on the(More)
Stretching helical strips of porcine a. carotis comm. to 1.7 times the resting length increases myosin light chain phosphorylation from 0.22 to 0.67 mol phosphate/mol light chain. While stretch is maintained, the stretch induced light chain phosphorylation decreases and reaches the resting level over a period of 60 minutes. The fully stretched arterial(More)
It has been well known that reperfusion following ischaemia may cause functional and structural damage to not only the organ involved but also the blood vessels supplying that organ. As in organ-sparing renal surgery it is inevitable to clamp the renal artery for some time, it is expected that reperfusion, following the removing of clamping, causes(More)
The mechanical and molecular dynamical properties of the glycerinated muscle fibres from m. psoas of rabbit were investigated after treatment with 5,5'-dithiobis/2-nitrobenzoate (DTNB). After DTNB treatment the fibre bundles shortened and exerted force in ATP-relaxing solution. The rate of the ATP hydrolysis in relaxation-inducing medium increased(More)
The Ca2+-binding gluten fraction isolated by the authors has been shown to prolong the clearing phase and to shorten the physiological contraction phase of ATP activated natural actomyosin suspension in the presence of physiological potassium chloride in a dose dependent manner. The mean ATPase activity of myosin and actomyosin was calculated for each phase(More)
OBJECTIVE We compared different signal transduction pathways through thyroid stimulating hormone receptor (TSH-R) in porcine thyroid cells (PTC) following stimulation with thyroid stimulating hormone (TSH) and 11 thyroid stimulating immunoglobulin samples (TSI) obtained from patients with Graves' disease. DESIGN Following stimulation with TSI, the level(More)