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Statistically significant differences were found to exist in the frequencies of abnormal cell types in cultured lenses depending on the composition of the nutrient media (Eagle's and Dulbecco's modified essential medium with fetal calf serum FCS) they were cultured on. Abnormal cell growth (epithelioid, fibroblast-like, infiltrating or bladder cells) was… (More)
This simple method is used for removing emulsion from unsuccessful histoautoradiographic preparations for putting a new layer of emulsion. For this purpose the old emulsion is solved in warm water, the silver residue is removed from the preparation surface with a 10% solution of potassium ferricyanide.
The epitheliomorphous zones of growth of piece explants of the mammalian embryonic lenses are comparable with those of neuroectodermal origin in formation of the rosette-shaped, perle-like and neuroretinal structures. They differ from the epidermal epithelia by absence of keratinization. The cambial and differentiated cells of the lens in the culture… (More)
Cultivated undifferentiated malignant nephroblastoma would produce fibroblastoid zones of growth. The tumor cells differed from normal renal fibroblasts by an abundance of mitochondria; cell boundaries were not distinguished. No differentiations in tumor cells cultures absent in the primary material were noted.
Retinas of 7-, 11- and 21-day-old turkey embryos were labelled with 3H-thymidine before their organotypic cultivation and in culture. It has been stated that at the beginning of cultivation the retinas of various ages preserve their histological structure. In immature retinas the nuclei continue their vertical migration with subsequent arrangement of… (More)
144 crystalline lenses of swine fetuses, 10 to 20 Mm long, were organ-cultured for up to 42 days in Eagles medium or DMEM in combination with 6% or 20% fetal calf serum. The types of cells detected included epithelioid and infiltrating cells (in 11.1% of lenses), fibroblast-like cells (in 20.8% of lenses) and vesicular cells (in 49.3% of lenses). These cell… (More)
The cells of S-phase labelled prior to cultivation with H3-thymidine and other neighbouring cambial cells of the lens of the pig, cattle and sheep were found to form morphologically underdifferentiated zones of growth. The zones of growth were formed in the culture from differentiating in vivo cells of the lens. The cells of these zones occasionally… (More)
Fraeser mouse lens morphology and potassium homeostasis were studied. It was shown that just at the age of one month mouse lens exposed "bull" cells which could be observed in patients with senile cataract as well. Nucleated fusiform extended cells were found 4 months later in the central part of the lens which was not typical for this part of the whole… (More)