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Journals and Conferences
Statistically significant differences were found to exist in the frequencies of abnormal cell types in cultured lenses depending on the composition of the nutrient media (Eagle's and Dulbecco's modified essential medium with fetal calf serum FCS) they were cultured on. Abnormal cell growth (epithelioid, fibroblast-like, infiltrating or bladder cells) was… (More)
Fraeser mouse lens morphology and potassium homeostasis were studied. It was shown that just at the age of one month mouse lens exposed "bull" cells which could be observed in patients with senile cataract as well. Nucleated fusiform extended cells were found 4 months later in the central part of the lens which was not typical for this part of the whole… (More)
Among human diseases that most often lead to blindness, the first place is occupied by cataract, or opacity of the lens of the eye . Although there may be different causes of different types of cataract, the mechanisms of development both of the commonest type of cataract, namely senile, and the less common hereditary type of cataract, are most probably… (More)
When the integrity of the fetal lens in tissue culture is disturbed it undergoes dedifferentiation and incomplete differentiation of the nucleated cells of the lens. Cell growths which form under these circumstances resemble the cellular masses of the lens affected by cataracts [1-3]. The question arises: what is the role of integrity of thelenticular… (More)
Retinas of 7-, 11- and 21-day-old turkey embryos were labelled with 3H-thymidine before their organotypic cultivation and in culture. It has been stated that at the beginning of cultivation the retinas of various ages preserve their histological structure. In immature retinas the nuclei continue their vertical migration with subsequent arrangement of… (More)
The epitheliomorphous zones of growth of piece explants of the mammalian embryonic lenses are comparable with those of neuroectodermal origin in formation of the rosette-shaped, perle-like and neuroretinal structures. They differ from the epidermal epithelia by absence of keratinization. The cambial and differentiated cells of the lens in the culture… (More)
144 crystalline lenses of swine fetuses, 10 to 20 Mm long, were organ-cultured for up to 42 days in Eagles medium or DMEM in combination with 6% or 20% fetal calf serum. The types of cells detected included epithelioid and infiltrating cells (in 11.1% of lenses), fibroblast-like cells (in 20.8% of lenses) and vesicular cells (in 49.3% of lenses). These cell… (More)
Cultivated undifferentiated malignant nephroblastoma would produce fibroblastoid zones of growth. The tumor cells differed from normal renal fibroblasts by an abundance of mitochondria; cell boundaries were not distinguished. No differentiations in tumor cells cultures absent in the primary material were noted.
This simple method is used for removing emulsion from unsuccessful histoautoradiographic preparations for putting a new layer of emulsion. For this purpose the old emulsion is solved in warm water, the silver residue is removed from the preparation surface with a 10% solution of potassium ferricyanide.