S. A. Lemmel

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Methanesulfonic acid ethyl ester (EMS) is effective in inducing mutants resistant to ampicillin, erythromycin, and butanol (15 g/l). Optimal mutagenesis occurs at 85–90% kill corresponding to a 15 minute exposure to 1.0% (v/v) EMS at 35 C. At optimal conditions, the frequency of resistant mutant CFU/ total CFU plated increases 100–200 fold.
The kinetics of growth and amylase production of Saccharomycopsis fibuligera were studied in a chemostat on a synthetic potato processing blancher water. Dilution rates (D) from 0.101 to 0.480 h were examined. A mathematical model based on the Monod equation was developed. The yield of cell mass from carbohydrates was constant and equal to 0.84. The maximum(More)
The yeasts Candida utilis and Saccharomycopsis fibuliger were propagated as a source of single-cell protein in a continuous, mixed, aerobic, single-stage cultivation on blancher water generated during potato processing. A series of steady-state experiments based on a two-level factorial design, half-replicate modified with an intermediate experiment, was(More)
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