S. A. Kosterin

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In experiments performed on digitonin-treated myometrium cells the existence of intracellular energy-dependent Ca(2+)-transporting system has been identified. The mitochondria-linked energy-dependent accumulation of Ca2+ was inhibited by ruthenium red and sodium azide with Ki of 0.7 +/- 0.3 microM and 1.1 +/- 0.4 mM, respectively. The Ca2+ ionophore A23187(More)
Using carbachol contracture as the experimental model for testing the properties of the intracellular calcium store in intact tissue and 45Ca2+ accumulation in the chemically skinned by digitonin smooth muscle cells isolated from oestrogen-dominated rat uterus the evidence for the presence of Mg2+, ATP-dependent Ca2+ pump in the non-mitochondrial store has(More)
Comparative investigation of sensitivity of calcium pumps of sarcolemma, endoplasmic reticulum and mitochondria of the smooth muscle to some inhibitors of energy-dependent Ca(2+)-transporting systems has been carried out in experiments made using the isotope method (45Ca2+) on the fraction of plasmatic membranes and suspension of chemically scanned cells of(More)
A method has been proposed for the kinetic analysis of the relaxation phase of mechanical response of smooth muscle. The method implies linearization of the entire mechano-kinetic relaxation curve using the coordinates ln[(fm-f)/f]; ln t (where fm and f are the maximal and the actual values of force within the relaxation phase respectively) with subsequent(More)
In order of estimating some regularities of ethanol direct (effectory) effect to transmembrane calcium metabolism in the myometrium the action of this substance on the energy-dependent Ca(2+)-transporting systems of the uterine myocytes subcellular structures has been studied. The systems of Mg2+, ATP-dependent Ca2+ transport regarding their sensitivity to(More)
Atom-absorption spectrophotometry have shown that the content of Ca2+ in the rabbit and cow myometrium amounts to 4.54 +/- 0.47 and 2.57 +/- 0.30 and that of Mg2+--3.89 +/- 0.15 and 1.35 +/- 0.17 mmol per 1 kg of wet tissue weight, respectively, The content of Mg2+ in the myometrium is two times lower than in the myocardium and three times lower than in the(More)
Some organic solvents (2-10%) have been comparatively studied for their effect on purified transporting Ca2+, Mg(2+)-ATPase, solubilized from the plasma membrane of smooth muscle cells and on actomyosine ATPase of the smooth muscle. The inhibiting effect of solvents on the initial maximum specific activity of Ca2+, Mg(2+)-ATPase corresponds to the sequence(More)
The supramolecular compound calix[4]arene C-90 (5,11,17,23-tetra(trifluoro)methyl(phenylsulfonylimino)-methylamino-25,26,27,28-tetrapropoxycalix[4]arene) is shown to efficiently inhibit the ATP hydrolase activity of Ca2+,Mg2+-ATPase in the myometrium cell plasma membrane fraction and also in a preparation of the purified enzyme solubilized from this(More)
The effects of temperature, dielectric permeability and ionic strength on the activity of purified Ca2+, Mg(2+)-ATPase solubilized from myometrial sarcolemma have been studied under saturation of the enzyme with Ca2+, Mg2+ and ATP. The values of activation energy calculated from Arrhenius plots for both ATP hydrolase reactions catalysed by solubilized and(More)