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Molecular Architecture and Functional Analysis of NetB, a Pore-forming Toxin from Clostridium perfringens*
TLDR
The crystal structure of the pore form of NetB is presented, showing that NetB does not bind phosphocholine efficiently but instead interacts directly with cholesterol leading to enhanced oligomerization and pore formation and providing potential new tools to the field of bionanotechnology. Expand
Molecular basis of toxicity of Clostridium perfringens epsilon toxin
TLDR
The exquisite specificity of the toxin for specific cell types suggests that it binds to a receptor found only on these cells, and the crystal structure of ε‐toxin reveals similarity to aerolysin from Aeromonas’hydrophila, parasporin‐2 from Bacillus’thuringiensis and a lectin from Laetiporus’sulphureus. Expand
Clostridium perfringens epsilon toxin H149A mutant as a platform for receptor binding studies
TLDR
The role of surface exposed tyrosine residues in domain I of E tx in binding to MDCK cells and the suitability of Etx‐H149A for further receptor binding studies are confirmed and have important implications for developing strategies designed to neutralise toxin activity. Expand
Clostridium perfringens epsilon toxin mutant Y30A-Y196A as a recombinant vaccine candidate against enterotoxemia
TLDR
Etx mutant Y30A-Y196A showed markedly reduced cytotoxicity towards MDCK and could form the basis of a recombinant vaccine against enterotoxemia. Expand
Identification of a Key Residue for Oligomerisation and Pore-Formation of Clostridium perfringens NetB
TLDR
Findings suggest that NetB K41H could be developed as a genetic toxoid vaccine to protect against necrotic enteritis and showed significantly reduced toxicity towards LMH cells and human red blood cells relative to wild type toxin. Expand
Protection against avian necrotic enteritis after immunisation with NetB genetic or formaldehyde toxoids☆
TLDR
Vaccination with a NetB genetic or formaldehyde toxoid protects chicken in an in vivo disease model and could form the bases of an efficient vaccine against necrotic enteritis. Expand
Variable protection against experimental broiler necrotic enteritis after immunization with the C-terminal fragment of Clostridium perfringens alpha-toxin and a non-toxic NetB variant
TLDR
The aim of this work was to determine whether immunization with a non-toxic variant of NetB and the C-terminal fragment of C. perfringens alpha-toxin would provide protection against experimental necrotic enteritis. Expand