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A polymorphic microsatellite (Y-27H39) based on a (GATA) n repeat was recently discovered on the short arm of the human Y chromosome. We have used a simple technique based on polymerase chain reaction amplification and native polyacrylamide gel electrophoresis followed by highly sensitive silver staining to study the inheritance, the genetic stability and(More)
  • S D Pena
  • 1980
Staining of fibroblast monolayers with Coomassie brilliant blue R250 provides a relatively easy and fast method for visualization of cytoskeletal elements. Staining of detergent-extracted monolayers improves resolution and provides preparations comparable in definition to those obtained by specific immunofluorescence.
Numerical chromosome abnormalities (aneuploidies) are among the most common known causes of mental retardation and the leading cause of pregnancy loss in humans. They primarily arise by the process of meiotic non-disjunction. We still know very little about the contribution of genetic and environmental causes for non-disjunction in humans. In order to(More)
The cc-chemokine receptor-5 gene protein (CCR5) is a seven-transmembrane-domain G protein coupled receptor that has as its natural ligands RANTES (regulated on activation normal T-cell expressed and secreted), MIP-alpha (macrophage inflammatory protein) and MIP-beta, which are members of the CC subfamily of chemokines (Samson et al., 1996a). The CCR5(More)
It is suggested that the mechanism of muscle-cell necrosis in various muscle diseases is explained by an increased net influx of calcium into cells which triggers a "vicious cycle" of mitochondrial calcium overloading and energy depletion. If correct, this hypothesis may offer the basis of a more rational treatment of some muscle diseases even before their(More)
Biotinyl derivatives of seven plant lectins-concanavalin A (Con A), peanut agglutinin (PNA), Ricinus communis agglutinin I (RCA I), Ulex europeus agglutinin I (UEA I), soybean agglutinin (SBA), Dolichos biflorus agglutinin (DBA), and wheat germ agglutinin (WGA)-were bound to cryostat sections of biopsied normal human muscle and visualized with(More)
A family of three generations has been described with an inser-tional type of chromosome rearrangement involving chromosomes 11 and 18 [46,XX or XY, ins(1l;l8)(pl5;ql1q21)] detected by G-banding using a trypsin digestion method. Four members ofthis family with clinical features of 18q-have inherited the der(l8) from their father and are thus deficient for(More)