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The experiments conducted on tonoplast of Beta vulgaris L. roots were performed to identify detergent-resistant lipid–protein microdomains (DRMs, interpreted as lipid rafts).The presence of DRMs can be found when dynamic clustering of sphingolipids, sterols, saturated fatty acids is registered, and the insolubility of these microdomains in nonionic(More)
257 According to the World Health Organization, approximately 2 billion people (that is, one third of the world population) are infected with hepatitis B virus (HBV), although there is a conventional yeasttbased parenteral vaccine against hepatitis B. Every year, 450–650 million people get infected with hepatitis B virus, with about 350 million acquiring(More)
Glutathione transferase (GST) activity revealed in vacuoles of red beetroot (Beta vulgaris L.) cells was investigated in comparison with the GST activity of plastids and extracts of tissues. The level of GST activity determined by spectrophotometric method proved fairly high in water extracts and membrane fractions of isolated vacuoles and plastids, as well(More)
To enhance translation of the L1 protein antigen of the oncogenic human papillomavirus type HPV16 L1, the Ω sequence of the 5'-untranslated region of tobacco mosaic virus was inserted into a genetic construct as an enhancer. To transform plants, A. tumefaciens EHA105 cells were transfected with this construct. After the genetic transformation, the HPV16 L1(More)
Results of investigation of peculiar properties related to the structure of plant cell vacuolar membranes are discussed. This investigation was conducted with the aid of confocal microscopy, which allowed us, in the process of scanning, to reveal nanotubes and vesicules in the preparations of isolated vacuoles. Such membrane nanotubes were observed both(More)
The presence of lipid-protein microdomains in biological membranes is considered proven in biological literature. The content of lipids and proteins in the microdomains depends on the membrane’s nature, as well as on the technique used for isolation of the membrane fractions. In the case of detergent technique (DT), the nativity of the microdomains (called(More)
Activity and isoenzyme composition of phenol-dependent vacuolar peroxidase (PO) were examined at different stages of development of red beet (Beta vulgaris L.) roots. The enzyme activity was found to increase during growth and decrease during the period of root dormancy. The isoenzyme composition of PO was also altered; additional cationic and anionic(More)
Changes in the enzymatic activity of phenol-dependent peroxidase (PO) of vacuoles and tissue extract of red beet (Beta vulgaris L.) roots in different phases of plant development and in hyperosmotic stress and pathogen infection were found. The highest activity was observed during root growth and the lowest PO activity occurred in dormancy, respectively.(More)
9 The group of proteins that are induced by the plant in stress includes peroxidase (EC 1.11.1.7) and polyphenol oxidase (EC 1.10.3) [1]. Peroxidase as a defense response protein has been attracting particular attention of researchers due to its high affinity for H 2 O 2 [1–4]. The greatest activity and diversity of perr oxidase isoforms are characteristic(More)
In the absence of H2O2, isoforms of vacuolar phenol-dependent peroxidase (PO) in beet (Beta vulgaris) roots oxidized phenolic compounds like tyrosinases. Tyrosinase activity of PO manifested a clearly expressed pH-dependence with the optimum at pH of 8.0–9.0; peroxidase activity was the highest at pH 5.0–7.0. The inhibitory analysis confirmed a specificity(More)