Ryuichi Kanagawa

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PURPOSE To evaluate the presence and distribution of lens epithelial cells (LECs) and extracellular matrix on intraocular lenses (IOLs) implanted in the capsular bag in rabbit eyes. SETTING Department of Ophthalmology, Wakayama Medical College, Wakayama, Japan. METHODS Five adult albino rabbits had phacoemulsification and IOL implantation in both eyes.(More)
Macrophages appearing on implanted intraocular lenses (IOLs) in the rabbit eye and in the mouse peritoneal space were observed using Wolter's implant cytology staining and scanning and transmission electron microscopy. In response to the implanted IOL, macrophages in the mouse peritoneal space displayed an activated form with marked ruffles on the surface.(More)
Transmission and scanning electron microscopy, Wolter's implant cytology staining, and an immunohistochemical method were used to investigate the process of cell adhesion, the origin of fibroblast-like cells, and the nature of lymphocytic clusters that were observed on intraocular lenses (IOLs) experimentally implanted in the rabbit eye and in the mouse(More)
Among the cell components appearing on an implanted intraocular lens (IOL), the macrophage is the most active participant in the foreign-body response. The morphological stages of the macrophage in the response include not only an activated form and a flat epithelioid cell, but also a giant cell observed in the later stage of the response. The giant cells(More)
We examined the effect of l-ascorbic acid 2-phosphate (P-Asc) on the proliferation of cultured rabbit keratocytes. P-Asc is a phosphate derivative of l-ascorbic acid and has more prolonged vitamin C activity in solution than does l-ascorbic acid. The proliferation of cultured keratocytes was promoted by the presence of P-Asc in culture medium. Transmission(More)
We investigated the origin of fibronectin (FN) on five posterior and four anterior chamber explanted intraocular lenses (IOLs) using immunohistochemical methods. Cellular deposits (assumed to be macrophages) and fibrous or membrane-like proteinaceous deposits on the IOLs showed immunoreactivity to an antibody against cellular FN. These proteinaceous(More)
An immunohistochemical study was performed to observe cellular proliferation on the surface of implanted intraocular lenses (IOLs) in rabbit eyes. Rabbits were killed at intervals of 3 days and 1, 2, and 4 weeks after the operation. The IOLs were removed and examined by an immunoperoxidase staining method using antifibronectin (anti-FN) antibodies. The FN(More)
Immunohistochemical studies of deposits were carried out on two intraocular lenses (IOLs) explanted from human eyes. One anterior chamber intraocular lens (AC-IOL) was studied using a monoclonal anti-human type I collagen-peptide antibody (C-Ab). One posterior chamber intraocular lens (PC-IOL) was studied using a monoclonal anti-human vimentin antibody(More)
The phagocytic activity and distribution of fibronectin in the cells adhering to implanted intraocular lenses (IOLs) were studied in rabbits. IOLs were explanted from the posterior chamber 7 days after implantation. Phagocytosis by the cells from the IOLs was studied by electron microscopy after incubation with polystyrene beads. The distribution of(More)
PURPOSE To use light microscopy to evaluate the presence and distribution of cells that proliferate on the outer surface of the anterior capsule after experimental lens extraction in rabbit eyes. SETTING Research Laboratory, Department of Ophthalmology, Wakayama Medical College, Wakayama, Japan. METHODS Extracapsular lens extraction, with or without(More)