Ryoji Nakaune

Learn More
New virus-like sequences, TvAQ7 and TvP15, were found in a Japanese grapevine accession of OKY-AQ7 (cv. Aki Queen) and of OKY-P15 (cv. Pione) by nested RT-PCR to simultaneously amplify a segment of the RNA-dependent RNA polymerase (RdRp) gene from members of the genera Vitivirus and Foveavirus. The TvAQ7 genome, except for an exact 5′ terminus, is composed(More)
Template preparation is important in reverse-transcription polymerase chain reaction (RT-PCR)-based detection methods. A TissueLyser with tungsten carbide beads was used for simultaneous processing of up to 48 samples under the same conditions in the development of a simple and rapid procedure to prepare a plant extract for RT reaction. A sandpaper method(More)
In Vitis labruscana ‘Pione’ and ‘Kyoho’, 57 grapevines with rugose wood (RW) symptoms and 31 asymptomatic grapevines were obtained from vineyards in Hiroshima and Okayama prefectures. We surveyed vines for RW-associated viruses and other viruses by reverse transcription-polymerase chain reaction with virus-specific primers and degenerate primers for(More)
Colletotrichum acutatum, the fungus causing anthracnose disease of various fruits and crops, has inherent resistance to benomyl. The mechanism underlying its resistance to benomyl remains unclear. We generated a benomyl-sensitive mutant CAT7-150 by transformation-mediated insertional mutagenesis. Subsequently, a CaBEN1 gene was isolated from CAT7-150 by(More)
Three viroid-like sequences were detected from Japanese persimmon (Diospyrus kaki Thunb.) by RT-PCR using primers specific for members of the genus Apscaviroid. Based on the sequences, we determined the complete genomic sequences. Two had 92.1–94.3% sequence identity with citrus viroid OS (CVd-OS) and 91.4–96.3% identity with apple fruit crinkle viroid(More)
The distribution of ABC (ATP-binding cassette) transporter genes among several taxonomically distinct phytopathogenic fungi was investigated by Southern blot hybridization, polymerase chain reaction assays and partial sequencing. Consensus sequences of the ABC transporter gene, which might be concerned with multidrug resistance against fungicides and with(More)
Two genes, BMR1 and BMR3, encoding new members of the ABC superfamily in Botrytis cinerea, were cloned and characterized. The topologies of the encoded proteins, BMR1 and BMR3, were both (NBF-TMD6)2, similar to most of the toxicant-efflux ABC transporters. These genes had different induced expression patterns after treatment with various toxicants,(More)
All grapevine leafroll-associated viruses (GLRaV) identified so far belong to the family Closteroviridae [9]. The genus Closterovirus includes GLRaV-2, and the genus Ampelovirus includes GLRaV-1 and -3 in subgroup I and GLRaV-4, including the genetically divergent variants GLRaV-5, -6, -9, -De, -Pr and -Car, in subgroup II [9]. The provisional genus(More)
A novel putative ampelovirus was detected in grapevines that showed typical leafroll symptoms and was tentatively named grapevine leafroll-associated virus (GLRaV)-13 following the series of numbering of other GLRaVs. The complete genome of GLRaV-13 comprised 17,608 nt and contained eleven putative open reading frames, showing genetic features similar to(More)