Ryo Hasegawa

Learn More
Erythropoietin (EPO) was successfully loaded on self-dissolving micropile array (SDMA) chip using chondroitin sulfate as the base polymer. "Drug glue" was prepared by adding EPO solution to chondroitin sulfate solution and SDMA was formed by micromolding fabrication technology. One SDMA chip, 1.0x1.0 cm, contained 100 micropile arrays. Two types of SDMA,(More)
Feasibility study of two-layered dissolving microneedles for percutaneous delivery of peptide/proteins using recombinant human growth hormone (rhGH) and desmopressin (DDAVP). Two-layered dissolving microneedles were administered percutaneously to the rat skin. Plasma rhGH and DDAVP concentrations were measured by EIA and LC/MS/MS. In vivo dissolution and(More)
Four new cardenolide monoglycosides, cardenolides N-1 (1), N-2 (2), N-3 (3), and N-4 (4), were isolated from Nerium oleander, together with two known cardenolides, 5 and 12, and seven cardenolide monoglycosides, 6-11 and 13. The structures of compounds 1-4 were established on the basis of their spectroscopic data. The in vitro anti-inflammatory activity of(More)
BACKGROUND This study tested the hypothesis that dissolving microneedles are a useful transdermal drug delivery system (TDDS) for insulin. METHODS Insulin was loaded on a patch (1.0 cm2) that had 100 dissolving microneedles with chondroitin sulfate by microfabrication technology. Pharmacodynamic evaluation was performed by applying two or four patches to(More)
Quinones can accept two electrons and two protons, and are involved in electron transfer and proton transfer reactions in photosynthetic reaction centers. To date, the pK a of these quinones in aqueous solution have not been reported. We calculated the pK a of the initial protonation (Q·− to QH·) and the second protonation (QH− to QH2) of 1,4-quinones using(More)
A requirements analysis step plays a significant role on the development of information systems, and in this step we produce various kinds of abstract models of the systems (called requirements models) according to the adopted development processes, e.g. class diagrams in the case of adopting object-oriented development. However, constructing these models(More)
Sixteen cardenolide triglycosides (1–16) were isolated from stems, twigs, and leaves of Nerium oleander. Among them, 3β-O-(4-O-gentiobiosyl-d-diginosyl)-7β,8-epoxy-14-hydroxy-5β,14β-card-20(22)-enolide, named cardenolide B-3 (16), was isolated from natural sources for the first time. The in vitro anti-inflammatory activities of compounds 1–16 were examined(More)
A new cardenolide diglycoside (1) was isolated from Nerium oleander together with ten known cardenolide diglycosides 2-11. The structure of compound 1 was established on the basis of their spectroscopic data. The in vitro anti-inflammatory activity of compounds 1-11 was examined on the basis of inhibitory activity against the induction of the intercellular(More)
OBJECTIVE We have performed laparoscopic adrenalectomy including retroperitoneoscopic adrenalectomy via a single large port (RASLP) and conventional laparoscopic adrenalectomy (CLA) for adrenal tumors since 1992, and report our experience to date. METHODS The study population consisted of 134 patients who underwent laparoscopic adrenalectomy from 1992 to(More)