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Empirical Mode Decomposition (EMD) provides a new method for analyzing signals from a nonlinear viewpoint. EMD is defined by an algorithm requiring experimental investigation instead of rigorous mathematical analysis. We show that EMD yields its own interpretation of combinations of pure tones. We present the problem of mode mixing and give a solution… (More)
An assay based on forward mutation of Dictyostelium discoideum to 3% methanol resistance allows quantitation of induced mutation following treatment with physical and chemical agents. Properties of this assay are: uniform recovery of methanol-resistant (MeOHr) cells over a wide range of plated cell densities, equal growth rates of methanol-sensitive and… (More)
We have cloned an AP endonuclease gene (APEA) from Dictyostelium discoideum, along with 1.8 kb of the 5' flanking region. There are no introns. The sequence predicts a protein of 361 amino acids, showing high homology to the major human/Escherichia coli exonuclease III family of AP endonucleases. There is 47% identity and 64% similarity to the Ape… (More)
The linkage of two mutations leading to increased sensitivity to ultraviolet light and 60Co gamma rays was determined in the slime mould Dictyostelium discoideum using a genetic analysis based on the parasexual cycle. Diploids were selected from a mixture of radiation-sensitive, temperature-resistant and radiation-resistant, temperature-sensitive haploids… (More)
The vegetative cells of the slime mold Dictyostelium discoideum are extremely resistant to cobalt-60 gamma rays. The survival curve has a shoulder at low doses and a 10 percent survival dose of 300 kilorads in air. Dose fractionation experiments indicate that repair of sublethal damage is an important factor in this resistance.