Learn More
In order to track the fate of HIV-1 particles from early entry events through productive infection, we developed a method to visualize HIV-1 DNA reverse transcription complexes by the incorporation and fluorescent labeling of the thymidine analog 5-ethynyl-2'-deoxyuridine (EdU) into nascent viral DNA during cellular entry. Monocyte-derived macrophages were(More)
Bone marrow transplantation is a curative treatment for many diseases, including leukemia, autoimmune diseases, and a number of immunodeficiencies. Recently, it was claimed that bone marrow cells transdifferentiate, a much desired property as bone marrow cells are abundant and therefore could be used in regenerative medicine to treat incurable chronic(More)
Efficient differentiation of embryonic stem cells (ESC) into hematopoietic progenitor cells (HPCs) is crucial for the establishment of stem cell-based therapies targeting the treatment of immunological and hematological disorders. However, so far, it has not been possible to induce long-term survival of murine ESC-derived HPCs without the overexpression of(More)
Passive Q switching of a flash-lamp-pumped ruby laser has been demonstrated with a Dy(2+):CaF(2) saturable absorber at room temperature. Typically a single Q-switched pulse of 4.5 mJ and 94 ns was observed. At 694 nm, the ground-state absorption cross section and the excited-state absorption cross section of the Dy(2+):CaF(2) absorber were found to be 1.2 ×(More)
We report what is to our knowledge the first dual operation comprising laser action and Q switching of Cr(4+):YAG pumped intracavity by a Nd:YAG laser. This technique produced dual 1.06- and 1.44-microm pulses with 35- and 200-ns pulse widths, respectively. The Nd:YAG rod was pumped by a 1-ms flash-lamp pulse, resulting in a train of 1.06- and 1.44-microm(More)
Passive Q switching of Er:glass (1.53 microm) with a new, robust, solid-state saturable absorber, Co(2+):Y(3)Sc(2)Ga(3)O(12), has been demonstrated. Q-switched pulses of 20 ns and 4 mJ of energy were obtained. An absorption cross section of 5 x 10(-20) cm(2) was measured. Preliminary results for a Co(2+):Y(3)Al(5)O(12) saturable absorber Q switch are also(More)
Macrophages are a target of human immunodeficiency virus type 1 (HIV-1) infection and may serve as an important reservoir of the virus in the body, particularly after depletion of CD4+ T cells in HIV/AIDS. Recently, sterile alpha motif and histidine/aspartic acid domain-containing protein 1 (SAMHD1) was identified as the major restriction factor of HIV-1(More)
  • 1