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Modern agriculture demands crops carrying multiple traits. The current paradigm of randomly integrating and sorting independently segregating transgenes creates severe downstream breeding challenges. A versatile, generally applicable solution is hereby provided: the combination of high-efficiency targeted genome editing driven by engineered zinc finger(More)
Targeted transgene integration in plants remains a significant technical challenge for both basic and applied research. Here it is reported that designed zinc finger nucleases (ZFNs) can drive site-directed DNA integration into transgenic and native gene loci. A dimer of designed 4-finger ZFNs enabled intra-chromosomal reconstitution of a disabled gfp(More)
The concept of information classification is used by all nations to control information distribution and access. In the United States this is referred to as Multiple Levels of Security (MLS), which includes designations for unclassified, confidential, secret, and top secret information. The U.S. Department of Defense has traditionally implemented MLS(More)
We present NetGrok, a tool for visualizing computer network usage in real-time. NetGrok combines well-known information visualization techniques—overview, zoom & filter, details on demand—with network graph and treemap visualizations. NetGrok integrates these tools with a shared data store that can read PCAP-formatted network traces, capture traces from a(More)
Targeted gene regulation via designed transcription factors has great potential for precise phenotypic modification and acceleration of novel crop trait development. To this end, designed transcriptional activators have been constructed by fusing transcriptional activation domains to DNA-binding proteins. In this study, a transcriptional activator from the(More)
The response regulator RpaA was examined by targeted mutagenesis under high light conditions in Synechocystis sp. PCC 6803. A significant reduction in chlorophyll fluorescence from photosystem I at 77 K was observed in the RpaA mutant cells under high light conditions. Interestingly, the chlorophyll fluorescence emission from the photosystem I trimers at 77(More)
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