Ruth Weaver Yokoyama

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The motility of cilia and flagella is powered by dynein ATPases associated with outer doublet microtubules. However, a flagellar kinesin-like protein that may function as a motor associates with the central pair complex. We determined that Chlamydomonas reinhardtii central pair kinesin Klp1 is a phosphoprotein and, like conventional kinesins, binds to(More)
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) causes nigrostriatal dopaminergic pathway damage similar to that observed in Parkinson disease (PD). To study the role of NO radical in MPTP-induced neurotoxicity, we injected MPTP into mice in which nitric oxide synthase (NOS) was inhibited by 7-nitroindazole (7-NI) in a time- and dose-dependent fashion.(More)
In Drosophila, dosage compensation-the equalization of most X-linked gene products between XY males and XX females-is mediated by the MSL complex that preferentially associates with numerous sites on the X chromosome in somatic cells of males, but not of females. The complex consists of a noncoding RNA and a core of five protein subunits that includes a(More)
We have isolated and sequenced genes from the blind cave fish, Astyanax fasciatus, that are homologous to the human red and green visual pigment genes. The data strongly suggest that, like human, these fish have one red-like visual pigment gene and multiple green-like visual pigment genes. By comparing the DNA sequences of the human and fish visual pigment(More)
We report here the isolation and sequence determination of a gene closely linked to the Astyanax red visual pigment gene. Reverse transcription polymerase chain reaction assays show that this new gene (B23 Af) and the previously characterized red and green visual pigment genes of Astyanax are all expressed in the eye. Phylogenetic analysis shows that B23Af(More)
Visual pigment genes have been isolated from a Mexican blind cave fish, Astyanax fasciatus. We report here the DNA sequence of one of these genes, which has 70% nucleotide similarity with both the human red and green pigment genes. This gene appears capable of encoding a functional protein and is probably responsible for the green-sensitive pigment found in(More)
The river-dwelling fishes of Astyanax fasciatus and their derivatives from Pachon and Micos caves in Mexico were collected. By comparing red (r007) and green (g103) opsin gene sequences from the three populations, we found a high frequency of C-->T changes in r007 and g103 of the eyed Micos fishes presumably due to spontaneous cytosine methylation or(More)
We previously found that a mutation at the ODA7 locus in Chlamydomonas prevents axonemal outer row dynein assembly by blocking association of heavy chains and intermediate chains in the cytoplasm. We have now cloned the ODA7 locus by walking in the Chlamydomonas genome from nearby molecular markers, confirmed the identity of the gene by rescuing the mutant(More)
Allelic differences at the alcohol dehydrogenase (ADH) and aldehyde dehydrogenase loci may have an important role in an individual's alcohol sensitivity. We have cloned and sequenced all nine exons of an ADH2(2) allele which codes for an 'atypical' ADH, ADH beta 2. Our sequence data shows that the histidine at residue 47 of ADH beta 2 is encoded by CAC.(More)
PURPOSE To isolate and characterize the rhodopsin cDNA from the fish, Astyanax fasciatus, and to determine the effect of tyrosine 261 on its spectral tuning. METHODS The rhodopsin cDNA was cloned using reverse transcription-polymerase chain reaction amplification and then sequenced. A mutant, Y261F, was generated by site-directed mutagenesis. Both wild(More)