Learn More
Between February 2011 and February 2012, 985 and 324 samples were collected from diarrheal and healthy pigs, respectively, to detect porcine epidemic diarrhea virus (PEDV), porcine kobuvirus (PKoV), porcine bocavirus (PBoV), porcine group A rotavirus (GARV), and transmissible gastroenteritis virus (TGEV). PEDV and PKoV clearly predominated in diarrheal(More)
Salmonella enterica serovar Choleraesuis strain C500 is a live, attenuated vaccine that has been used in China for over 40 years to prevent piglet paratyphoid. We compared the protective efficacies of subcutaneous (s.c.) and oral vaccination of BALB/c mice with C500 expressing the recombinant filamentous hemagglutinin type I domain and pertactin region 2(More)
The aim of this study was to determine the genetic diversity of Chinese feline calicivirus (FCV) isolates and their phylogenetic relationship with isolates from elsewhere in the world. Phylogenetic analysis was performed based on the partial open reading frame (ORF) 2 sequences (regions B-F) of 21 Chinese FCV isolates and 30 global isolates. The Chinese(More)
With the widespread penetration of broadband accesses, many multimedia applications, such as video surveillance, on-demand video streaming, IPTV and news broadcast are emerging. Since video streaming applications on the Internet generally have very high bandwidth requirements and yet are often unresponsive to network congestion. In order to avoid congestion(More)
The success of peer-to-peer overlay multicast for media streaming depends on their scalability. However, as multicast functionality is pushed to autonomous, unpredictable end systems, significant performance loss can be resulted from their higher degree of churn when compared to routers. Data distribution path over an overlay consisting of unreliable peers(More)
In this paper, we present a receiver-based, bandwidth estimation rate control mechanism with content-aware probability retransmission to limit the burden on multimedia transmission congested network. Like the TCP friendly rate control (TFRC) protocol, we compute the sending rate as a function of the loss event rate and round-trip time. Considering the(More)
Bordetella bronchiseptica is a Gram-negative respiratory pathogen responsible for atrophic rhinitis and bronchopneumonia in swine. Several vaccines aimed at preventing B. bronchiseptica have been used, but a safe and efficient live vaccine for use in piglets remains elusive. In this study, we constructed an aroA-deleted B. bronchiseptica strain (QH0814) and(More)
OBJECTIVE We evaluated the efficacy of the recombinant pertactin (PRN)-specific active or passive immunization against Bordetella bronchiseptica (Bb) in an aerosol challenge model established by using BALB/c mice. METHODS AND RESULTS Mice, immunized subcutaneously with two doses of purified glutathione S-transferase (GST)-PRN protein mixed with an equal(More)
OBJECTIVE Glutamyl tRNA Synthetase (Gts) is a protease which catalyzes esterification between tRNA and glutamine. The immunogenicity of Gts was evaluated through immunization and challenge experiment. METHODS AND RESULTS We cloned gts from the genome of SC19, and inserted it into prokaryotic expression plasmid pET28a-gts. The recombinant vector was(More)
OBJECTIVE We developed an indirect ELISA method for detecting Bordetella bronchiseptica (Bb) pertactin antibodies based on the recombinant pertactin protein expressed in Escherichia coli (DE3) strain. METHODS AND RESULTS The prn gene encoding Bb pertactin was fused to the downstream of glutathione S-transferase (GST) of pGEX-KG expression vector,(More)