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The major pterin in Tetrahymena pyriformis, strain W, earlier suggested to be L-threo-biopterin and named ciliapterin [1] is now identified as D-threo-neopterin (D-monapterin). This is the first example of a natural D-monapterin. This compound was characterized by its chromatographic behavior, its fluorescence properties and by its oxidation product with(More)
Optically active lumazines (biolumazine, dictyolumazine, monalumazine, and neolumazine) are prepared from the corresponding pterins by enzymatic reaction, using pterin deaminase excreted by Dictyostelium discoideum. The fluorescence properties, circular dichroism spectra, and chromatographic behavior of these lumazines are studied. D- and L-enantiomers of(More)
Lumazine has been demonstrated to be one of the two main compounds responsible for the extracellular fluorescence linked with the aggregation ability of Dictyostelium discoideum, strain Ax-2. The other compound, also having lumazine properties, is, however, different from the 7-hydroxylumazine proposed previously. The influence of pH on the fluorescence of(More)
A major pterin was isolated by reverse-phase high-performance liquid chromatography from cellular extract of vegetative cells of Dictyostelium discoideum after perchloric deproteinization and oxidation with acidic iodine. This compound was characterized by its chromatographic behavior, its absorption and fluorescence properties, by its oxidation product(More)
A simple method was developed for the isolation of the cationic endopeptidase from a crude extract prepared from the culture medium of Thermoactinomy ces vulgaris, consisting in chromatography on Sephadex G 75 and subsequent separation on CM-Sephadex in 50 mM Tris/HCl, pH 8, using a NaCl-gradient (0 - 0,2 M). This procedure results in a 4,2 fold increase of(More)
Intracellular dictyopterin, which is present in reduced form, mainly as tetrahydrodictyopterin, both in vegetative cells and in starved cells, could neither be detected in dormant spores nor in unswollen or swollen activated spores. Synthesis of tetrahydrodictyopterin starts with emergence of nascent amoebae during spore germination. Tetrahydrodictyopterin(More)
The separation of D- and L-enantiomers of 6-(polyhydroxypropyl)pterins was obtained by ligand-exchange chromatography using a reversed-phase column at 12 degrees C with a mobile phase containing D-phenylalanine as the chiral modifier and Cu(II) as the metal ion. This allowed the determination of the stereoconfiguration of natural pterins from very small(More)