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We report the development of a novel procedure for generating fast, high-frequency Ca2+ oscillations in vitro and the frequency-dependent activation of m-calpain, the Ca2+-activated intracellular cysteine protease. The procedure is based upon liberating Ca2+ from a cage, DM-Nitrophen, by repetitive UV laser pulses and its concomitant binding by a 'slow'(More)
Our objective was to develop a simple procedure for the detection of light-induced ion currents of photomotile cells in two dimensions. The novel technique was based on the light gradient method (LGM), and the model object was Chlamydomonas reinhardtii, a phototactic unicellular alga, ideal for such experiments. The conventional LGM cuvette was modified(More)
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