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Surface plasmon resonance spectroscopy and quartz crystal microbalance study of streptavidin film structure effects on biotinylated DNA assembly and target DNA hybridization.
Surface plasmon resonance (SPR) spectroscopy is employed for the study of biotinylated DNA assembly on streptavidin modified gold surfaces for target DNA hybridization. Two immobilization strategiesExpand
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Detection of point mutation and insertion mutations in DNA using a quartz crystal microbalance and MutS, a mismatch binding protein.
MutS protein is a mismatch binding protein that recognizes mispaired and unpaired base(s) in DNA. In this study, we incorporate the MutS protein-based mutation recognition into quartz crystalExpand
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Transcriptional regulation by antitermination. Interaction of RNA with NusB protein and NusB/NusE protein complex of Escherichia coli.
A recombinant heterodimeric NusB/NusE protein complex of Escherichia coli was expressed under the control of a synthetic mini operon. Surface plasmon resonance measurements showed that theExpand
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Crystal structures of the antitermination factor NusB from Thermotoga maritima and implications for RNA binding.
NusB is a prokaryotic transcription factor involved in antitermination processes, during which it interacts with the boxA portion of the mRNA nut site. Previous studies have shown that NusB exhibitsExpand
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Multiplexed hybridization detection of quantum dot-conjugated DNA sequences using surface plasmon enhanced fluorescence microscopy and spectrometry.
In this study, the general suitability of quantum dot (QD)-DNA conjugates for the surface plasmon enhanced fluorescence spectroscopy technique is demonstrated. Furthermore, the QD-DNA system isExpand
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Imaging of G protein-coupled receptors in solid-supported planar lipid membranes
This contribution summarizes first some of our efforts in imaging G-protein-coupled receptor (GPCR) functional inserted into planar tethered lipid bilayer membranes (tBLMs) as a novel platform forExpand
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Surface plasmon resonance spectroscopy and quartz crystal microbalance study of MutS binding with single thymine-guanine mismatched DNA.
MutS is a DNA mismatch binding protein that recognizes heteroduplex DNA containing mispaired or unpaired bases. In this study, we employ a quartz crystal microbalance (QCM) and a surface plasmonExpand
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Oligonucleotide hybridization monitored by surface plasmon enhanced fluorescence spectroscopy with bio-conjugated core/shell quantum dots. Influence of luminescence blinking
The influence of the luminescence blinking of quantum dots on kinetic bio-sensing measurements is investigated. The hybridization kinetics of labelled DNA sequences with surface-immobilized DNAExpand
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RNA DNA discrimination by the antitermination protein NusB.
The regulation of ribosomal RNA biosynthesis in Escherichia coli by antitermination requires binding of NusB protein to a dodecamer sequence designated boxA on the nascent RNA. The affinity of NusBExpand
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In vitro Synthesis of GPCRs in a Solid Supported Planar Lipid Membrane System
Membrane protein research depends largely on the availability of functional protein in an instrumental set- up for application of biophysical investigations. We used an in vitro synthesis strategy inExpand