Ruby N I Pietersz

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BACKGROUND AND OBJECTIVE Buffy coat (BC) pooling sets are integrated systems, consisting of a pooling bag, a filter and a platelet storage container, for the production of leucoreduced platelet concentrates (LR-PCs) from pooled BCs. It was our aim to compare various pooling sets that are currently marketed. MATERIALS AND METHODS LR-PCs were made by(More)
BACKGROUND Based on real-time polymerase chain reaction (PCR) technology, a broad-range 16S rDNA assay was validated and its performance was compared to that of an automated culture system to determine its usefulness for rapid routine screening of platelet concentrates (PCs). STUDY DESIGN AND METHODS The presence of bacteria in pooled PCs was routinely(More)
The leukocyte depletion capacity and performance of 5 filters designed for filtration of red cell concentrates (RCC) were compared by counting leukocytes, measuring red cell volumes and by histological examination of the filters after use. To eliminate interdonor differences, 5 buffy-coat-poor RCC were pooled (in each of 10 experiments) and subsequently(More)
BACKGROUND AND OBJECTIVES Whole blood can be separated by hard spin centrifugation into layers of blood components according to their specific gravity. The aim was to develop a program for an automatic separator to subsequently express the various components into their respective satellite bags in top and bottom systems with the following requirements: a(More)
A method is described to prepare platelet concentrates from the buffycoat of citrate-phosphate-dextrose (CPD) blood in a closed four-bag system and to store the platelets in autologous plasma under sterile conditions. After separation of the blood into plasma, buffycoat and leukocyte- and thrombocyte-poor red cell concentrate,(More)
Transfusion of leukocyte-free red cell concentrates (RCC) prevents or delays HLA immunization in multitransfused patients. We investigated a new cellulose acetate filter which was recently introduced to remove leukocytes from buffy-coat-poor RCC. It was found that the filtration time was only 10 min with buffy-coat-poor RCC in(More)
A flow cytometric method for the detection of low amounts of lymphocytes, monocytes, and granulocytes in filtered red cells (RBCs) was evaluated. In this procedure, the RBCs in the samples were lysed by ammonium chloride treatment and the white cells (WBCs) were detected by flow cytometry according to their specific light-scattering properties. The identity(More)
The activation of platelets during the preparation of platelet concentrates (PCs) by two methods was compared. To eliminate interdonor differences, 2 units of whole blood were pooled and subsequently divided into two batches. From one batch, the platelets were harvested as pelleted platelets from platelet-rich plasma (PRP) and from the other as nonpelleted(More)
The purpose of the present overview was to determine the factors influencing the removal of infectious agents from red cell concentrates by filtration. In general, the efficacy of the filtration method depends on the physical as well as the functional properties of blood cells. These properties are highly influenced by the changes exerted on the blood cells(More)
BACKGROUND Storage of PLT concentrates (PCs) may be extended beyond 5 days, provided in-vitro and in-vivo variables allow longer storage and bacterial screening is performed. The aim of this study was to examine in-vitro storage characteristics of PCs in various storage solutions: plasma only, or mixtures of plasma with PAS-II, PAS-III, PAS-IIIM, and(More)