Rubén Durán

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Trypanosoma cruzi, the causative agent of the Chagas disease, has a complex life cycle alternating between replicative and noninfective forms with nonreplicative and infective forms of the parasite. Metacyclogenesis is a process that takes place in the invertebrate host, comprising morphogenetic transformation from a noninfective form to an infective form,(More)
Nitrile hydratase from Brevibacterium sp. R312 was purified to homogeneity. The isoelectric point was 5.75. The two kinds of subunits were separated by reverse phase HPLC and their N-terminal amino acid sequences were found to be identical to those of Rhodococcus sp. N-774 nitrile hydratase.
Replication of several cryptic plasmids from coryneform strains was investigated in Brevibacterium sp. R312. Only the Corynebacterium glutamicum pSR1 replicon was found to be suitable for establishing a host-vector system. Two pSR1 derivatives, pRPCG200 and pHYCG1, were used as cloning vectors. They carry a neomycin-resistance-encoding and a(More)
Sorosporium texanum was described by ZUNDEL almost 25 years ago (6) from a single known collection made by Mr. HANSEL in Brownsville, Texas on Pennise tum nervosum (NEES) TRIN. (Coll. No. 52794, National Fungus Collection, through communication with J. A. STEVENSON). ZUNDEL apparently never collected this species during his career nor is it known to have(More)
Mating experiments between monosporidial lines ofSorosporium consanguineum from Mexico and the Pacific Northwest indicate that incompatibility in theAristida smut is controlled by multiple alleles at one locus. Alleles a1 a2 were demonstrated in a collection from Chihuahua, Mexico onAristida ternipes. It is hypothesized that these alleles also control(More)
Promoter sequences recognized by Escherichia coli RNA polymerase were isolated from Brevibacterium sp. R312, a coryneform strain producing nitrile hydratase and amidase. Ten Escherichia coli clones containing promoter sequences were selected for their ability to grow with chloramphenicol concentrations of up to 1500 micrograms/ml. The strength of these(More)
Saprophytic development of Sorosporium consanguineum and its nuclear cycle were studied on laboratory media. During vegetative reproduction, the nuclei of the monokaryotic sporidia were shown to first migrate into developing bud cells where division occurs, one nucleus returning to the parent cell prior to completion of cell division. Following fusion of(More)
To clone the malolactic enzyme gene from Lactobacillus sp. 89, construction of a shuttle vector able to express itself in Lactobacillus sp. 89 and Escherichia coli was undertaken. The shuttle plasmid pLE16 resulted from the union of pBR328 and of the pLB10 plasmid extracted from Lactobacillus bulgaricus 10. The bacterial transformation in Lactobacillus sp.(More)
Monosporidial lines ofSorosporium consanguineum Ell. & Ev. isolated from a smut collection made in the Pacific Northwest onAristida longiseta Steud. were pathogenic only when cultures with contrasting alleles for incompatibility were paired and used to inoculate seedlings ofA. longiseta. Moreover, in pairing these lines with monosporidial lines from another(More)
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