Rozamond Y Sweeney

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Assays for Ribonucleolytic Activity 81 A sensitive and convenient assay for catalytic activity is an essential tool for studying catalysis. Because of the seminal role of bovine pancreatic ribonuclease A (RNase A; EC in the history of enzymology, 1 assays for ribonucle-olytic activity have existed for decades. The recent discovery of ribonucleases(More)
Ribonuclease A (RNase A) catalyzes the cleavage of RNA after pyrimidine nucleotides. When bound in the active site, the base of a pyrimidine nucleotide forms hydrogen bonds with the side chain of Thr45. Here, the role of Thr45 was probed by using the wild-type enzyme, its T45G variant, X-ray diffraction analysis, and synthetic oligonucleotides as ligands(More)
2'-Fluoro-2'-deoxyuridine 3'-phosphate (dU(F)MP) and arabinouridine 3'-phosphate (araUMP) have non-natural furanose rings. dU(F)MP and araUMP were prepared by chemical synthesis and found to have three- to sevenfold higher affinity than uridine 3'-phosphate (3'-UMP) or 2'-deoxyuridine 3'-phosphate (dUMP) for ribonuclease A (RNase A). These differences(More)
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