Rositsa Denkova

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OBJECTIVE To clarify the in vitro effects of inhibin A (I) on apoptotic cell death and its mechanisms in ovarian granulosa cells the immunoexpression patterns of the apoptosis markers caspase-3 and pro- and anti-apoptotic proteins (Bcl-2, Bcl-xl, Bak) were evaluated in ovarian granulosa cells collected from women with different hormonal status. MATERIALS(More)
Possible involvement of microtubules in the regulation of granulosa cell steroidogenesis by large follicle granulosa cell conditioned media (LGCCM) was assessed by monitoring the effect of agents that alter the cytoplasmic microtubule-tubulin equilibrium. The changes in microtubule organization and cell shape were examined by immunohistochemical procedure(More)
OBJECTIVE Nitric oxide (NO) is involved in different cell functions including ovarian steroid production. Endothelin-1 (ET-1) was found to regulate the steroidogenesis in ovarian granulosa cells (GC). The present study was designed to receive more information about the mechanism of action of NO in the process of ET-1 induced progesterone (P) inhibition,(More)
OBJECTIVE To investigate the in vitro effect of endothelin-1 (ET-1) on the steroid production (progesterone [P] and estradiol [E2]) by cultured human granulosa cells (GCs) during aging. MATERIAL AND METHODS Human ovarian GCs and granulosa-luteal cells (GLCs) were isolated from ovaries of female patients (young and premenopausal) undergoing surgery for(More)
Investigations were carried out into the activity and localization of NADH-dependant diaphorase in boar spermatozoa. Semen samples were collected from healthy boars, used in A.I. centers. The enzyme was extracted with distilled water and Triton X-100. Two forms of diaphorase were found-water-soluble and Triton X-100 soluble, showing low activity-0.36 U/ml(More)
Examination of the technological properties of newly isolated strains of the genus Lactobacillus and possibilities for their application in the composition of starters The ability of four Lactobacillus strains À Lactobacillus brevis LBRZ7 (isolated from fermented cabbage), Lactobacillus plantarum LBRZ12 (isolated from fermented cabbage), Lactobacillus(More)
The ability of porcine granulosa cells to release a progesterone inhibiting substance(s) was examined in vitro. Granulosa cells (SGCs, MGCs and LGCs) were harvested from small, medium or large antral follicles respectively. The effect of granulosa cell conditioned media obtained from small follicles (SGCCM) was studied in the culture of LGCs by estimation(More)
The present study was undertaken to investigate the possible involvement of prostanoids in the inhibitory effect of endothelin-1 (ET-1) on progesterone production of ovarian granulosa cells. ET-1 (1 x 10(-7) M) decreased the basal and follicle-stimulating hormone- (FSH) stimulated progesterone production from both human and porcine granulosa cells.(More)
OBJECTIVE To extend our previous observations of granulosa cell conditioned medium inhibition on basal and FSH-stimulated progesterone secretion by granulosa cells from large porcine antral follicles. METHODS Granulosa cell conditioned media (SGCCM) were obtained from cultures of granulosa cells which were harvested from small porcine antral follicles.(More)