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Intracellular free [Ca2+] ([Ca2+]i) was imaged in Fura-2 loaded human umbilical vein endothelial cells, using dual excitation light, an inverted epifluorescence microscope and a silicon intensified target camera. Oscillations of [Ca2+]i were induced in these cells by 0.5 microM histamine. Because the image processor contained a large random access memory of(More)
The advent of time-frequency methods have opened a whole new chapter in sonar signal processing. Current implementations are based on conventional techniques like FFT etc, which have many shortcomings. Although the Fourier transform yields perfect reconstruction of a broad class of signals, it does not necessarily provide a meaningful interpretation when(More)
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