Roshelle Silverman

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Formation of terminal synapses at sites such as the neuromuscular junction involves transformation of the motile growth cone into the nonmotile synaptic terminal. However, transformation does not need to be the mechanism when a neurite forms multiple widely spaced synaptic varicosities along a target in an en passant configuration. Synaptic varicosities(More)
Novel macromolecular therapeutics such as peptides, proteins, and DNA are advancing rapidly toward the clinic. Because of typically low oral bioavailability, macromolecule delivery requires invasive methods such as frequently repeated injections. Parenteral depots including biodegradable polymer microspheres offer the possibility of reduced dosing frequency(More)
Peripheral injections of the tracer fluorogold (FG) and immunocytochemistry were used to study the modulation of gonadotropin-releasing hormone (GnRH) cell secretory activity in adult mice. Intraperitoneal administration of FG would make it available to all GnRH terminals outside the blood-brain barrier. The degree of capture of the dye would be linked to(More)
The homozygous mutant hypogonadal (hpg) mouse lacks a functional gene for the neuropeptide gonadotropin releasing hormone (GnRH). The consequence of this defect is an infantile reproductive tract in adulthood. This condition can be reversed by the implantation of normal fetal preoptic area tissue that contains GnRH neurons. Reversal is always preceded by(More)
When immunoreactive human parathyroid hormone (hPTH), extracted by three different solvents (20% acetone in 1% acetic acid, 8 M urea, or normal saline) from parathyroid glandular tissue was subjected to Sephadex G-100 gel filtration and immunoassay using two different antisera (273 and C-329), four distinct fractions were observed. The first (I), a void(More)
The mutant hypogonadal (hpg) mouse lacks a functioning gene for the neurohormone gonadotropin releasing hormone (GnRH). Previous studies from our laboratory had indicated that the initiation and maintenance of reproductive function in these mice could be brought about by the implantation of normal fetal grafts into adult hosts. Testicular or ovarian growth(More)
The hypogonadal (hpg) mouse lacks GnRH due to a severe truncation of the gene by which it is encoded. This results in an infertile animal with an infantile reproductive system. When fetal or 1-day postnatal septal/preoptic area of a normal mouse is grafted into the third ventricle of an hpg mouse, GnRH-containing fibers grow out of the grafts and innervate(More)
Transplantation of brain tissue has been used to ameliorate the genetic lesion of the hypogonadal mutant mouse. This animal does not synthesize gonadotropin-releasing hormone (GnRH) and so has an infantile reproductive system. Implantation of normal fetal or neonatal preoptic area containing GnRH neurons reverses many aspects of the reproductive deficiency.(More)
The precursor protein that contains the sequence for the neurohormone gonadotropin releasing hormone (GnRH) also contains an additional fragment (amino acids 14-26, designated pHGnRH14-26) that can release luteinizing hormone (LH) and follicle stimulating hormone (FSH) in vitro. An immunocytochemical study was carried out to determine if this sequence could(More)