Roselyne Boulieu

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BACKGROUND Ketamine is used as an anaesthetic agent for short surgical procedures, and as a sedative and analgesic in intensive care patients. Intensive care patients with brain or spinal cord injury may have physiological changes that could alter the pharmacokinetics of ketamine. The pharmacokinetics of ketamine have been studied in healthy volunteers and(More)
Ketamine is a widely used drug for its anesthetic and analgesic properties; it is also considered as a drug of abuse, as many cases of ketamine illegal consumption were reported. Ketamine is N-demethylated by liver microsomal cytochrome P450 into norketamine. The identification of the enzymes responsible for ketamine metabolism is of great importance in(More)
6-thioguanine (6-TGN) and methyl 6-mercaptopurine nucleotides (Me6-MPNs) are the two major metabolites found in erythrocytes after administration of azathioprine. In an attempt to understand the role of these metabolites in the pharmacologic and toxic activity of thiopurines, we have developed a HPLC method for the simultaneous determination of 6-TGNs and(More)
BACKGROUND Polymorphic thiopurine S-methyltransferase (TPMT) is a major determinant of thiopurine toxicity. METHODS We extracted 6-thioguanine nucleotides (6-TGNs) and 6-methylmercaptopurine nucleotides (6-MMPNs) from erythrocytes with perchloric acid and converted them to 6-thioguanine (6-TG) and a 6-methylmercaptopurine (6-MMP) derivative during a(More)
This study aimed at assessing the effects of midazolam (MDZ) sedation on auditory brainstem (BAEP) and middle latency (MLAEP) evoked potentials in intensive care conditions. Ten ventilated comatose patients were receiving an intravenous MDZ bolus dose (0.2 mg/kg) followed by a 2 h continuous infusion (0.1 mg/kg/h). MLAEPs and BAEPs elicited by clicks (90 dB(More)
A simple, fast, precise and sensitive method for the quantification of IR3535 ® , an insect repellent used in topical formulations, was developed by reversed-phase high performance liquid chromatography. The separation was carried out by using a core-shell silica particles column (Kinetex ® C18, 5 m, 4.6 mm x 150 mm) with a mobile phase consisting of 0.1%(More)
2010 platform (Roche Diagnostics). Ionized calcium and 25-hydroxyvita-min D (Nichols Advantage) were also measured in all samples. Results for the healthy individuals are presented in Fig. 1. The bio-intact PTH values measured by the IRMA and chemiluminescence immunoas-say were, on average, 50% and 30% lower, respectively, than the PTH measured by the(More)
Tartrate-resistant acid phosphatase 5b: a novel serum marker of bone resorption. Development of a kinetic assay for band 5b tartrate-resistant acid phosphatase activity in serum. Novel serum markers of bone resorption: clinical assessment and comparison with established urinary indices. Iron transfer between purple phosphatase uteroferrin and transferrin(More)
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