Rosalba Maci

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Dysfunction of the microtubule system is emerging as a contributing factor in a number of neurodegenerative diseases. Looking for the potential role played by the microtubule cytoskeleton in neuron degeneration underlying Parkinson's disease (PD), we investigate the influence of the parkinsonism producing neurotoxin 1-methyl-4-phenylpyridinium (MPP+) on(More)
Recent results have shown that apoptosis is an important feature of the normal and injured lung epithelium, but little conclusive evidence is available about the exact intracellular mechanisms involved. In this work, we studied apoptotic cell death in the established human lung epithelial cell line, A549, by evaluating the ability of the pulmonary toxin,(More)
The microtubular system is emerging as a cell target in neurodegeneration evoked by the Parkinsonism-inducing neurotoxin N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and its toxic metabolite N-methyl-4-phenylpyridinium (MPP(+)). Looking for a direct effect of the neurotoxin on microtubules, we have undertaken an in vitro study by using microtubule(More)
Nitric oxide (NO) is a signaling molecule implicated in a spectrum of cellular processes including neuronal differentiation. The signaling pathway triggered by NO in physiological processes involves the activation of soluble guanylate cyclase and S-nitrosylation of proteins, and, as recently proposed, nitration of tyrosine residues in proteins. However,(More)
The herbicide paraquat (PQ) induces the selective necrosis of type I and type II alveolar pneumocytes. We investigated the effect of PQ on human lung A549 cells to determine the possible role of cytoskeleton in lung cytotoxicity. At 80 μmol/L PQ, a concentration that did not affect cell viability, the organization of actin cytoskeleton network depended on(More)
In vivo, the neurotoxin MPTP is oxidated to MPP+, which is toxic to dopaminergic neurons. In this paper, we have used MPP+ as a tool to evoke neurotoxicity in the PC12 cell line and investigate the intracellular events that are involved. A cytotoxicity test, performed on undifferentiated and NGF-differentiated PC12 cells, showed that MPP+ is much more toxic(More)
Nitric oxide (NO) is a precursor of reactive nitrating species, peroxynitrite and nitrogen dioxide, which modify proteins to generate oxidized species such as 3-nitrotyrosine that has been used as a hallmark of peroxynitrite-mediated oxidative stress on proteins. In the last few years however, a growing body of evidence indicates that NO also regulates a(More)
Indirect immunofluorescencc, rhodamine-phalloidin staining and immunoelectron microscopy performed with the on-grid postembedding immunostaining of Lowicryl K4M sections, were used to identify actin in the branchial epithelium of the lower chordate ascidians. The ciliated cells of these invertebrates present two distinct junctional patterns. One consists(More)
L'actinomicina D, la puromicina e il LiCl producono, agendo nei primi stadi dello sviluppo dell'embrione di polio, malformazioni caratteristiche differenti nei 3 casi. I diagrammi di sedimentazione di preparati di ribosomi dimostrano l'azione dell'actinomicina D e della daunomicina sulla sintesi del RNA e della puromicina sulla sintesi proteica. Il LiCl(More)
Tyrosine nitration of proteins is emerging as a post-translational modification playing a role in physiological conditions. Looking for the molecular events triggered by nitric oxide in nerve growth factor-induced neuronal differentiation, we now find that nitration occurs on the microtubule-associated protein tau. In differentiated PC12 cells, we have(More)