Rong-yao Chai

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An F8 recombinant inbred population was constructed using a commercial indica rice variety Zhong 156 as the female parent and a semidwarf indica variety Gumei 2 with durable resistance to rice blast as the male parent. Zhong 156 is resistant to the fungus race ZC15 at the seedling stage but susceptible to the same race at the flowering stage. Gumei 2 is(More)
A pair of near isogenic lines G205 and G71 were selected from recombinant inbred lines (RIL) of Zhong156 x Gumei2. On the resistance locus Pi-25(t), G205 had the resistant allele that was from Gumei 2 while G71 had the susceptible allele that was from Zhong156. For the genetic background, different alleles were detected on only 24 loci out of the 672 RFLP(More)
The peroxisomal matrix proteins involved in many important biological metabolism pathways in eukaryotic cells are encoded by nucleal genes, synthesized in the cytoplasm and then transported into the organelles. Targeting and import of these proteins depend on their two peroxisomal targeting signals (PTS1 and PTS2) in sequence as we have known so far. The(More)
Site-specific variable pesticide application is one of the major precision crop production management operations. Rice blast is a severe threat for rice production. Traditional chemistry methods can do the accurate crop disease identification, however they are time-consuming, require being executed by professionals and are of high cost. Crop disease(More)
In a recombinant inbred line (RIL) population of indica rice, two subpopulations composed of susceptible lines were selected for mapping of partial resistance to leaf blast with two isolates of the pathogen. A third subpopulation composed of susceptible lines with similar heading time was used for mapping of partial resistance to neck blast with a third(More)
The family members of PEX11 are key factors involved in regulation of peroxisome proliferation. Sixty-six PEX11p candidates of PEX11 gene family from 26 representative fungal species were obtained and analyzed by bioinformatic strategies. In most filamentous fungi, 2 or 3 potential PEX11ps were found, in contrast with 1 or 2 in yeast species. Compared with(More)
The reflectance of rice that infects different severity leaf blasts was measured through artificial inoculation and disease index (DI) of the rice corresponding to the spectra which were acquired in the field. The correlation between DI and the first derivative data was analyzed. The estimation models of DI were built using regression methods, and RMSE were(More)
Two cDNA libraries enriched for transcripts differentially expressed in plants of two rice lines with similar genetic backgrounds and same leaf blast resistance but different responses to neck blast using suppression subtractive hybridization (SSH). After differential screening and sequence analysis of the selected clones, 90 unique cDNA clones were found,(More)
Targeted gene replacement (TGR) is an important technique for gene function analysis. With the development of genome sequencing and transformation, TGR has been applied widely to filamentous fungi, and many new systems and approaches have been established. In this paper, strategies involved in TGR in filamentous fungi were reviewed, including transformation(More)
In this study, differentially expression genes were isolated from a indica rice cultivar Zhong156 with a race-specific blast resistance gene subjected to the avirulent race ZC15 and the virulent race ZB1 of Magnaporthe grisea at the seedling growth stage. Twenty-five cDNA clones were isolated by using suppression subtractive hybridization combined with(More)
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