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Malachite green (MG), a recalcitrant, carcinogenic, and mutagenic triphenylmethane dye, was decolorized and detoxified using crude manganese peroxidase (MnP) prepared from the white rot fungus Irpex lacteus F17. In this study, the key factors (pH, temperature, MG, Mn(2+), H2O2, MnP) in these processes were investigated. Under optimal conditions, 96 % of 200(More)
PURPOSE To investigate bystander effects of low-dose-rate (LDR) (125)I seed irradiation on human lung cancer cells in vitro. METHODS AND MATERIALS A549 and NCI-H446 cell lines of differing radiosensitivity were directly exposed to LDR (125)I seeds irradiation for 2 or 4 Gy and then cocultured with nonirradiated cells for 24 hours. Induction of(More)
The optimization of existing sewer systems or making new systems is and will remain one of the key issues in drainage management in our society. The problem consists of minimization of a nonlinear cost function subjected to nonlinear constraints. To overcome the difficulties of the optimization of drainage systems, in this paper, an elitist adaptive genetic(More)
The effects of increasing lead concentration on the activities of superoxide dismutase (SOD), peroxides (POD) and catalase (CAT), levels of ascorbate (AsA), reduced glutathione (GSH), Pb accumulation and its influence on nutrient elements, polyamines (PAs) content, as well as activities of polyamine oxidase (PAO) and ornithine decarboxylase (ODC), were(More)
A hydroponic experiment was conducted to study the subcellular distribution of Cd and mineral elements in Alternanthera philoxeroides leaves and the leaves anti-oxidative capacity and chlorophyll, soluble protein, and phytochelatins (PCs) contents under the stress of different concentration (0, 0.05, 0.1, 0.2, 0.4 mmol x L(-1)) Cd. With the increasing(More)
The substitution box (S-box) is found in many block cipher, and it is a very important component. Since the chaotic system has several significant advantageous properties desirable for cryptosystem, the design of S-box using chaos has attracted a great deal of attention in recent years. In this paper, a novel method for designing key-dependent S-boxes based(More)
The growth of Irpex lacteus F17 and manganese peroxidase (MnP) production in a selfdesigned tray bioreactor, operating in solid-state conditions at a laboratory scale, were studied. The bioreactor was divided into three layers by three perforated trays. Agroindustrial residues were used both as the carrier of bound mycelia and as a nutrient medium for the(More)
White-rot fungus manganese peroxidase (MnP) oxidizes a wide range of substrates, rendering it an interesting enzyme for potential applications. The stability of MnP can be improved by immobilization. With sodium alginate, gelatin, or chitosan as a carrier, and glutaraldehyde as the crosslinking agent, MnP was co-immobilized using the embed-crosslinked(More)
OBJECTIVE To provide effective microorganisms for the treatment of water polluted by microcystins (MCs), the strains capable of biodegrading microcystin LR (MC-LR) were isolated from the sediment of Chaohu Lake. The degradation of microcystin-LR by the intracellular extracts of the strains were studied. METHODS The enrichment culture using MC-LR as the(More)
BACKGROUND Manganese peroxidase (MnP) from Irpex lacteus F17 has been shown to have a strong ability to degrade recalcitrant aromatic pollutants. In this study, a recombinant MnP from I. lacteus F17 was expressed in Escherichia coli Rosetta (DE3) in the form of inclusion bodies, which were refolded to achieve an active enzyme. Further, we optimized the in(More)