Ronald T. Plessinger

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Cultures of allograft sites on burn patients occasionally show microbes that were not previously cultured from that patient. Our purpose was to determine 1) if microbes on allograft sites could have been transferred to the burn patient from the allograft donor and 2) if microbial transfer is different if the allograft was fresh or frozen. All allografts(More)
Cadaveric human skin provides an optimal temporary cover after early excision of full-thickness burns; however, engraftment is reduced greatly by cryopreservation. Refrigerated skin is generally preferred because of its rapid revascularization, presumably caused by its greater viability. In this study, the effects of storage solutions, temperature, and the(More)
Adult nude mice had 1.5 to 1.0 meshed split-thickness human skin applied to an excised area of their back. The animals were then randomized into two groups, one of which had a steroid cream applied to their graft on alternate days. The other group had no ointment applied and served as a control. The wounds were measured on a weekly basis and the rate of(More)
Aquaphor Gauze was evaluated as a dressing for skin graft donor sites, for partial thickness burn injuries and for split thickness skin grafts. Control dressings consisted of: fine mesh gauze for skin graft donor sites. silver sulphadiazine (Silvadene) on coarse mesh gauze for the partial thickness burns, and nitrofurazone cream (Furacin) on fine mesh gauze(More)
A LMC micromethod using specific B lymphocyte target cells labeled with a fluorescent dye has been developed to select matched donors and recipients for kidney allotransplantation. The fluorescence of the B lymphocyte target cells was 85% or less of control levels in 9 of 28 patients. In 8 of these 9 hosts graft loss occurred. Nineteen sets of target cells(More)
Skin autografting was first described by Reverdin in 1871 [1]. The clinical utility of allograft skin as a method for wound coverage followed shortly thereafter [2]. Girdner [3] was the first to report the use of allogeneic skin to cover a burn wound; however, it wasn’t until 5 years later that Thiersch [4] described the histologic anatomy of skin(More)
P. aeruginosa proliferates well in a water environment; however, when subjected to high doses of streptomycin or gentamicin, the residual viable bacteria are killed by moderate water dilution of their media. These results lead to the suggestion that the mechanism of lethal action of aminoglycosides may operate through interference with the water balance(More)
A direct, specific and rapid method has been developed to prospectively evaluate lymphocyte mediated cytotoxicity (LMC) for kidney transplant recipients. Donor lymphocytes, labeled with fluorescein diacetate, were incubated in a microtest plate for 2 h with recipients' effector cells. The percentage of fluorescent cells in each well was estimated relative(More)
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