Roman Kubínek

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Atomic force microscopy (AFM) as one the technique of Scanning Probe Microscopy is useful for imaging of surface structure. This method can yield three-dimensional high-resolution topographic images of sample surfaces by using a scanning technique for conductors and insulators on atomic scale. It is based upon mapping of atomic-forces on a surface of an(More)
Photodynamic therapy is a modality of treatment for tumors. The photochemical interactions of sensitizer, light and molecular oxygen produce reactive oxygen species (ROS) such as singlet oxygen, peroxide, hydroxyl radical and superoxide ion. The tumor is destroyed either by the formation of highly reactive singlet oxygen (type II mechanism) or by the(More)
Dentin of human teeth is a vital hydrated tissue. It is strongly sensitive to dehydration and drying that are commonly used in preparation of samples for scanning electron microscopy. Experience in examination of dentine surfaces of extracted human third molars using contact mode atomic force microscopy under moist conditions is described. The examined(More)
Department of Experimental Physics, Faculty of Science, Palacký University, Olomouc, Czech Republic 1st Clinic of Stomatology, Medical Faculty, Palacký University, Olomouc, Czech Republic Department of Microscopy methods, Medical Faculty, Palacký University, Olomouc, Czech Republic Department of Medical Biophysics, Medical Faculty, Palacký University,(More)
There are many kinds of dendritic crystal types in nature. For example, we can adduce fern leafs or lovely dendritic crystallized snowflakes. Dendritic crystallization can be observed with iron under special conditions, not only salt solutions building typical dendritic crystals in the viscous environment. It is suitable to have sufficient tools capable of(More)
The crystallization in mixture of physiological solution (0.9% NaCl) with human albumin (model system) or blood serum leads to characteristic dendritic crystals. The composition of blood serum influences crystallization results in dependence on simultaneous pathological process of the organism. The crystallographic method is based on comparison and(More)
There are many possible approaches for direct image classification of dendritic crystals. Dendritic crystallization can be observed by iron in special conditions; common examples are fern leafs or dendritic crystallized snowflakes. This contribution is especially focused on the new structural classification methods we used (Hough transform, fractal(More)
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