Author pages are created from data sourced from our academic publisher partnerships and public sources.
- Publications
- Influence
Revealing Nature’s Cellulase Diversity: The Digestion Mechanism of Caldicellulosiruptor bescii CelA
- Roman Brunecky, Markus Alahuhta, +9 authors Y. Bomble
- Chemistry, Medicine
- Science
- 20 December 2013
An Enzyme Drill Cellulase enzymes degrade the cell walls of plants by breaking down cellulose into its constituent sugar fragments and thus have attracted interest for biofuels production. Using… Expand
Heterologous expression of glycosyl hydrolases in planta: a new departure for biofuels.
The concept of expressing non-plant glycosyl hydrolase genes in plant tissue is nearly two decades old, yet relatively little work in this field has been reported. However, resurgent interest in… Expand
High-Throughput Screening Techniques for Biomass Conversion
- S. Decker, Roman Brunecky, M. Tucker, M. Himmel, M. J. Selig
- Environmental Science
- BioEnergy Research
- 14 October 2009
High-throughput (HTP) screening of biomass or biomass-degrading enzymes, regardless of the desired outcome, is fraught with obstacles and challenges not typically faced in more traditional… Expand
Probing the role of N-linked glycans in the stability and activity of fungal cellobiohydrolases by mutational analysis
The filamentous fungi Trichoderma reesei and Penicillium funiculosum produce highly effective enzyme mixtures that degrade the cellulose and hemicellulose components of plant cell walls. Many fungal… Expand
In planta expression of A. cellulolyticus Cel5A endocellulase reduces cell wall recalcitrance in tobacco and maize
- Roman Brunecky, M. J. Selig, +4 authors S. Decker
- Biology, Medicine
- Biotechnology for biofuels
- 26 January 2011
The glycoside hydrolase family 5 endocellulase, E1 (Cel5A), from Acidothermus cellulolyticus was transformed into both Nicotiana tabacum and Zea mays with expression targeted to the cell wall under a… Expand
Cellulase Linkers Are Optimized Based on Domain Type and Function: Insights from Sequence Analysis, Biophysical Measurements, and Molecular Simulation
- Deanne W. Sammond, Christina M. Payne, Roman Brunecky, M. Himmel, M. Crowley, G. Beckham
- Biology, Medicine
- PloS one
- 6 November 2012
Cellulase enzymes deconstruct cellulose to glucose, and are often comprised of glycosylated linkers connecting glycoside hydrolases (GHs) to carbohydrate-binding modules (CBMs). Although linker… Expand
Cel48A from Thermobifida fusca: Structure and site directed mutagenesis of key residues
- M. Kostylev, M. Alahuhta, +5 authors D. Wilson
- Chemistry, Medicine
- Biotechnology and bioengineering
- 1 April 2014
Lignocellulosic biomass is a potential source of sustainable transportation fuels, but efficient enzymatic saccharification of cellulose is a key challenge in its utilization. Cellulases from the… Expand
ORIGINAL RESEARCH: Lignocellulose recalcitrance screening by integrated high-throughput hydrothermal pretreatment and enzymatic saccharification
- M. J. Selig, M. Tucker, +5 authors S. Decker
- Chemistry
- 6 May 2010
Abstract We report a novel 96-well multiplate reactor system for comparative analysis of lignocellulose recalcitrance via integrated hydrothermal pretreatment and enzymatic saccharification. The… Expand
Charge engineering of cellulases improves ionic liquid tolerance and reduces lignin inhibition
- Erik M. Nordwald, Roman Brunecky, M. Himmel, G. Beckham, J. Kaar
- Chemistry, Medicine
- Biotechnology and bioengineering
- 1 August 2014
We report a novel approach to concurrently improve the tolerance to ionic liquids (ILs) as well as reduce lignin inhibition of Trichoderma reesei cellulase via engineering enzyme charge.… Expand
Sequence, Structure, and Evolution of Cellulases in Glycoside Hydrolase Family 48*
- L. O. Sukharnikov, Markus Alahuhta, +4 authors I. Zhulin
- Biology, Medicine
- The Journal of Biological Chemistry
- 10 October 2012
TLDR