Rolf Kleine

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Cyclodextrin glycosyltransferase (CGTase) was produced by a 3-day cultivation of Bacillus macerans growing in a natural medium containing grated-potatoes. Besides CGTase the culture supernatant contained a mixture of serine proteinases with a predominant subtilisin-like activity. By fractionated precipitation with ammonium sulphate the CGTase (molecular(More)
  • Rolf Kleine
  • Zeitschrift für vergleichende Physiologie
  • 2004
Hepatopancreas (HP) of the crayfishes Astacus astacus (L.) and Cambarus affinis (Say) contains a high molecular carboxypeptidase A like action (substrate: carbobenzoxyglycyl-l-phenylalanine) (K d-value on Sephadex G-200 =0,04), an arylamidase like action (substrate: l-leucine-β -naphthylamide·HCl; LNA), and a dipeptidase (substrate: glycyl-l-leucine) (K(More)
The exopeptidase and dipeptidase activities of the hepatopanereas and gastric juice of Astacus astacus (L.) and Cambarus affinis (Say) were determined (mean values from approximately 90 exemplares). The carboxypeptidase which was highly active in the gastric juice hydrolyzes carbobenzoxyglycyl-l-phenylalanine and carbobenzoxy-l-glutamyl-l-tyrosine at about(More)
Some strictures are given on the problem of random sampling and interpretation in the photometry from the view of statistics. There is laid great store by the appointment that the problem is one of regression calculus of second manner, since both the argument and the ordinates mostly are stochastic variables. A numeric example to demonstrate the computing(More)
  • Rolf Kleine
  • Zeitschrift für vergleichende Physiologie
  • 2004
The esterase activities of hepatopancreas extracts and gastric juice from the crayfish Astacus astacus (L.) and Cambarus affinis (Say) were determined quantitatively. Astacus-hepatopancreas contains the highest activities. It is 10 to 23 times more active than Cambarus-hepatopancreas and 171 to 247 times more active than gastric juice of the two species.(More)
N a c h A u f t r a g e n yon 0 ,5 -1 ml E x t r a k t au f Pap ie r ( S & S 2043b mgl) w u r d e n die PC abs t e igend m i t I s o b u t t e r s g u r e E i s e s s ig -Wasse r ( 1 9 : 0 , 4 : t t ) [3,g] einoder zweimal 2 0 h bet I R a u m t e m p e r a t u r entwickel t . Mit P h o t o p r i n t v e r f a h r e n [g] wa ren au t den PC m a x i m a l 14(More)
Seven microbial peptide inhibitors—chymostatin, antipain, elastatinal, leupeptin, pepstatin, bestatin, and phosphoramidon—were tested for their efficiency to inhibit thermitase, a thermostable serine protease fromThermoactinomyces vulgaris. Chymostatin and antipain were the most effective inhibitors, with Ki values of 7×10−8 M and 2×10−7 M, respectively.(More)
  • Rolf Kleine
  • Zeitschrift für vergleichende Physiologie
  • 2004
Es wird über die Hydrolyse synthetischer Peptidsubstrate (Aminosäureester und -amide, Peptidester und acylierte Peptide) durch Organhomogenate von Fischen (Engraulis enchrasicholus, Mullus barbatus, Belone akus, Mugil auratus) und Mollusken (Rapana bezoar, Mytilus galloprovincialis) des Schwarzen Meeres berichtet. Die untersuchten Fischorgane (insbesondere(More)
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