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In the intact, in vitro frog skin, isoproterenol (ISO) stimulates an amiloride-insensitive increase in short-circuit current (SCC) that can be localized to the exocrine glands and is associated with secretion of chloride. To determine which cells in the glands respond to stimulation we measured the intracellular electrolyte concentrations of the various(More)
For elucidation of the functional organization of frog skin epithelium with regard to transepithelial Na transport, electrolyte concentrations in individual epithelial cells were determined by electron microprobe analysis. The measurements were performed on 1-micron thick freeze-dried cryosections by an energy-dispersive X-ray detecting system.(More)
The technique of electron microprobe analysis was used to determine the intracellular electrolyte concentrations in proximal or distal tubular cells of the rat kidney during ischemia. When the exposed kidney was maintained in air during ischemia, the composition of the surface cells differed little from control, and the electrolyte disturbances were(More)
Electron microprobe analysis of quick-frozen distal colonic epithelium from guinea pig was used to locate the cells responding to secretory stimuli. Concentrations of Na, K, and Cl were similar for cells of surface and crypt in the unstimulated state, 8, 149, and 46 mmol/kg wet weight, respectively. Stimulation of either K and Cl secretion with(More)