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Percent aggregation and the aggregation rate of platelet rich plasma (PRP) in response to ristocetin (1.75 mg/ml) were measured in 20 normals and 16 patients with von Willebrand's disease (v Wd), with and without the addition of acetylsalicylic acid (ASA). Percent aggregation did not clearly distinguish between normals and patients with vWd. Aggregation… (More)
Washed platelets were ruptured by freezing and thawing; a coagulant activity was released which would correct the clotting time of factor XI (FXI)-deficient plasma only in the presence of kaolin. Platelets from a FXI-deficient patient treated in a similar fashion also released a coagulant activity which could be absorbed onto Sepharose-heparin and eluted… (More)
Antibodies were raised in rabbits against purified human factor XII and insolubilized on Sepharose. This preparation of insolubilized anti-factor XII antibody was used to prepare factor-XII-free human platelet-rich and plate-poor plasma for use in the study of platelet coagulant activities.