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Full-length DNA copies of both B- and M-RNA of cowpea mosaic virus (CPMV) were constructed downstream from a T7 promoter. By removal of nucleotides from the promoter sequence, B- and M-RNA-like transcripts with varying numbers of additional nonviral sequences at the 5' end were obtained upon transcription with T7 RNA polymerase. The infectivity of the(More)
The fundamental characteristics of a distributed computing environment are heterogeneity, partial failure, latency and difficulty of " gluing together " multiple, independent processes into a robust, scalable application. JavaSpaces, which is a shared memory paradigm, provides high-level coordination mechanism for Java easing the burden of creating(More)
Using a full-length cDNA clone of cowpea mosaic virus (CPMV) B-RNA from which infectious transcripts can be generated, we examined the influence of a sequence of 11 nucleotides, UUUUAUUAAAA, comprising the nucleotides 5883 to 5893 in the 3' noncoding region of B-RNA, on viral RNA replication. This sequence is not only present in B-RNA but also in M-RNA and(More)
A set of full-length DNA copies of both M and B RNA of cowpea mosaic virus (CPMV) was cloned downstream of a phage T7 promoter. Upon in vitro transcription using T7 RNA polymerase, M and B RNA-like transcripts were obtained from these DNA copies with only two additional nucleotides at the 5' end and five extra nucleotides at the 3' end in comparison to(More)
Software development is proceeding at a remarkable rate. Many new tools are available to the researcher in parallel and distributed processing. These tools include PVM, MPI, and Java. But, recently, a more general tool, Common Object Request Broker Architecture, (CORBA) has appeared. Since it allows a general view of remote object invocation, and hence(More)
With multiprocessor computers becoming more readily available on college campuses and with the recent development of software tools to aid in the implementation of parallel algorithms, it is time for parallel processing to enter the undergraduate curriculum. In this paper we show how widely available tools combined with concepts learned in an operating(More)
With the resurgence of interest in distributed processing and programming of distributed systems, researchers are exploring methods and techniques for facilitating programming of such systems. In this paper we demonstrate the ease of use and performance characteristics of socket communication for scripting languages PERL and Python. Productivity is enhanced(More)
Networked Unix workstations as well as workstations based on Windows 95 and Windows NT are fast becoming the standard for computing environments in many universities and research sites. In order to harness the tremendous potential for computing capability represented by these networks of workstations many new (and not so new) tools are being developed.(More)