Rofina Yasmin Othman

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Embryogenic callus was established using immature male flower of Musa acuminata cv. Mas. After 5–6 months of culture, embryogenic callus was obtained at 21.75±11.9 from 750 immature male flower clusters with translucent somatic embryos proliferated from the whitish friable callus. It was observed that flower clusters ranging from 4 to 11 responded to form(More)
MS (Murashige and Skoog) media supplemented with benzylaminopurine (BAP) showed that the number of bud formation in shoot cultures of Musa acuminata cv.Berangan during the initiation stage increased proportionately with the concentrations used (11, 22 and 33 μM). However, the highest concentration of BAP (33 μM) simultaneously increased the formation of(More)
Suckers collected from different populations of Musa acuminata ssp. malaccensis were found to be highly resistant to race 4 of Fusarium oxysporum f. sp. cubense (FOC) suggesting that local wild banana populations co-evolved with the pathogen. Seedlings from these wild banana plants segregated for resistance to the pathogen. The infected seedlings were(More)
RNA extraction from seaweed tissues is problematic due to the presence of polysaccharides and polyphenolic compounds upon cell disruption. Besides, a successful RNA isolation from seaweed tissues can sometimes be strain- and species-specific. Four different methods were used to extract RNA from Gracilaria changii (Gracilariales, Rhodophyta), collected from(More)
Using a Biolistic PDS 1000/He system, healthy thalli of Gracilaria changii were bombarded with gold particles coated with plasmid DNA containing the lacZ reporter gene. Transient expression of lacZ was observed in bombarded thalli under the rupture-disc pressures of 4482, 6206, 7584 and 8963 KPa, two days after bombardment. Although G. changii exhibits a(More)
Type II (proteic) toxin-antitoxin systems (TAS) are ubiquitous among bacteria. In the chromosome of the pathogenic bacterium Streptococcus pneumoniae, there are at least eight putative TAS, one of them being the yefM-yoeB(Spn) operon studied here. Through footprinting analyses, we showed that purified YefM(Spn) antitoxin and the YefM-YoeB(Spn) TA protein(More)
Morphological identification of fish taxa can sometimes prove difficult because phenotypic variation is either being affected by environmental factors, phenotypic characters are highly conserved or marker selection has been inappropriate. DNA based markers especially neutral mitochondrial DNA (mtDNA) have been used widely in recent times to provide better(More)
Camilla J. Beech1, S.S. Vasan1,2, M. Megan Quinlan3, Margareth Lara Capurro4, Luke Alphey1,5, Vicente Bayard6, Madama Bouaré7, Maria Corena McLeod8, Pattamaporn Kittayapong9, James V. Lavery10,11, Lee Han Lim12, Mauro Toledo Marrelli13, J. Nagaraju14, Kenneth Ombongi15, Rofina Yasmin Othman16,17, Vilasini Pillai18, Janine Ramsey19, Rachel Reuben20, Robert(More)
The present study established a protocol for generating embryogenic cell suspension (ECS) cultures from an early stage embryogenic callus of Musa acuminata cv. ‘Berangan’. The necessary baseline data for morphohistological recognition and the selected gene expression for differentiating embryogenic and non-embryogenic cells were defined to enable early(More)
Wood can be a good source of DNA for various applications in forensic forestry and timber trade if high-quality DNA can be retrieved from the dry wood. In order to provide a general guideline for DNA authenticity testing established for Neobalanocarpus heimii, this study was designed to evaluate the potential of extracting DNA from the dry wood. Overall,(More)