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Total lipids were extracted from food products by heating the sample under total reflux for 15 hr with a mixture of chloroform--methanol (70 + 30) in the ratio 6.67 mL solvent/g sample. The solution was filtered and evaporated, and the lipids wer dissolved in petroleum ether, filtered, and evaporated to yield the crude lipids and surfactants. The purified(More)
A new and simplified procedure for the automated determination of serum alkaline phosphatase uses the AutoAnalyzer. The substrate p-nitrophenylphosphate in 2-amino-2-methyl-l-propanol offers the prime advantage of providing directly its own chromogen, p-nitrophenol, following enzyme action. The procedure also permits use of small sample volumes, provides(More)
Guidance of electrophysiological (EP) procedures by magnetic resonance imaging (MRI) has significant advantages over x-ray fluoroscopy. Display of electroanatomic mapping (EAM) during an intervention fused with a prior MR volume and DE-MRI derived tissue classification should improve the accuracy of cardiac resynchronization therapy (CRT) for ventricular(More)
An automated procedure is presented for the enzymatic determination of serum uric acid on both the AutoAnalyzer and the Robot Chemist. The procedure measures as the neocuproine complex, the difference in the amount of Cu+ formed by reaction of a Cu+++ aIkanoIamine buffered solution with serum uric acid under precisely controlled conditions before and after(More)
A precise automated procedure developed for determination of serum lactate dehydrogenase, using the Robot Chemist, measures absorbance of a cuprous-neocuproine complex formed by coupled reduction of the cupric-neocuproine reagent with enzymatically generated reduced nicotinamide adenine dinucleotide. Activity values obtained by this method, by the identical(More)
Catheter ablation of ventricular tachycardia (VT) is preceded by characterization of the myocardial substrate via electroanatomical voltage mapping (EAVM). The purpose of this study was to characterize the relationship between chronic myocardial fibrotic scar detected by multicontrast late enhancement (MCLE) MRI and by EAVM obtained using an MR-guided(More)
A new, precise, and accurate procedure is described for the automated determination of serum alkaline phosphatase on the Robot Chemist. The substrate, p-nitrophenyl-phosphate in 2-amino-2-methyl-1-propanol, offers the prime advantage of providing directly its own chromogen, p-nitrophenol, following enzyme action. Other advantages are the small sample(More)
Four cases of Mendelson's syndrome (acid pulmonary aspiration) are presented. They all demonstrate an acute diffuse alveolar filling pattern. This appearance is by no means specific. However, in the absence of other causes for this pattern and of evidence of left ventricular failure the radiologist may alert the clinician to the correct diagnosis. Early(More)