Rodrigo Martinez

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Bacillus subtilis strains are used for extracellular expression of enzymes (i.e., proteases, lipases, and cellulases) which are often engineered by directed evolution for industrial applications. B. subtilis DB104 represents an attractive directed evolution host since it has a low proteolytic activity and efficient secretion. B. subtilis DB104 is hampered(More)
Previously, we reported the presence in mouse cells of a mitochondrial RNA which contains an inverted repeat (IR) of 121 nucleotides (nt) covalently linked to the 5' end of the mitochondrial 16S RNA (16S mtrRNA). Here, we report the structure of an equivalent transcript of 2374 nt which is over-expressed in human proliferating cells but not in resting(More)
This paper presents our experience implementing three approaches for the 'PAN 2014 Author Identification' [3,1] task using the same representation for the author's style. Two of our approaches extend previous successful approaches: naive Bayes [4] and impostor [8] methods. The third approach is based on original research on sparse representation for text(More)
In this work we will study the problem of the design of the "Integration Model for Distributed Database System". We particularly design the canonical model through the ontological handling of the information. The ontology is designed in a way that allows the description of a database like a set of representative terms of its different components. In this(More)
We present a description of our approach for the " Bird task Identification LifeCLEF 2014 ". Our approach consists of four stages: (1) a filtering stage for the filtering of audio bird recordings; (2) segmentation stage for the extraction of syllables; (3) a candidate generation based on HOG features from the syllables using SVM; and (4) a species(More)
We report the isolation of Histoplasma capsulatum from a culture of the viscera of Didelphis albiventris, one of the marsupial species found in Brazil. To our knowledge, this is the first report of the isolation of this fungus from this mammalian species. This finding confirms the ubiquitous presence of H. capsulatum in nature.
Chemoenzymatic cellulose degradation is one of the key steps for the production of biomass-based fuels under mild conditions. An effective cellulose degradation process requires diverse physico-chemical dissolution of the biomass prior to enzymatic degradation. In recent years, “green” solvents, such as ionic liquids and, more recently, deep eutectic(More)
Recombinant protein production in prokaryotic and eukaryotic organisms was a key enabling technology for the rapid development of industrial and molecular biotechnology. However, despite all progress the improvement of protein production is an ongoing challenge and of high importance for cost-effective enzyme production. With the epMEGAWHOP mutagenesis(More)
Ultrahigh throughput screening (uHTS) plays an essential role in directed evolution for tailoring biocatalysts for industrial applications. Flow cytometry-based uHTS provides an efficient coverage of the generated protein sequence space by analysis of up to 10(7) events per hour. Cell-free enzyme production overcomes the challenge of diversity loss during(More)
Esterases hydrolyze ester bonds with an often high stereoselectivity as well as regioselectivity and are therefore industrially employed in the synthesis of pharmaceuticals, in food processing, and in laundry detergents. Continuous screening systems based on p-nitrophenyl- (e.g., p-nitrophenyl acetate) or umbelliferyl-esters are commonly used in directed(More)