Rodney J. Roughley

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Inoculation of legume seed is an efficient and convenient way of introducing effective rhizobia to soil and subsequently the rhizosphere of legumes. However, its full potential is yet to be realised. Following widespread crop failures, the manufacture of high quality inoculants revolutionised legume technology in Australia in the 1960s. Many improvements to(More)
p a ge 1. I n t r o d u c t i o n . . . . . . . . . . . . . . . . . . . . . . . 675 2. P r e p a r a t i o n of the peat carrier . . . . . . . . . . . . . . . . 676 3. P r e p a r a t i o n of broth cultures . . . . . . . . . . . . . . . . . 681 4. C o n d i t i o n s of g r o w f h in the fermenter . . . . . . . . . . . . 682 5. M i x i n g of the broth(More)
Strain ANU1173 is an acid-tolerant Rhizobium leguminosarum biovar trifolii strain that is able to nodulate subterranean clover plants growing in agar culture at pH 4.4 At pH 6.5, its symbiotic effectiveness in association with Trifolium subterraneum cv. Mt. Barker was 80% relative to that of strain ANU794, a Sm derivative of the commercial inoculant R.(More)
The effect of rice culture on changes in the number of a strain of soybean root-nodule bacteria, (Bradyrhizobium japonicum CB1809), already established in the soil by growing inoculated soybean crops, was investigated in transitional red-brown earth soils at two sites in south-western New South Wales. At the first site, 5.5 years elapsed between the harvest(More)
Morphological changes that take place in peat cultures of several species of rhizobia were examined. These changes seemed to be associated with enhanced survival of cells in peat and after inoculation onto plastic beads, which were used as a model system for seeds. Cell wall changes, in which the periplasmic space appeared to be occluded with electron-dense(More)
The coding regions of the human decorin, biglycan and fibromodulin cDNAs have been examined utilizing the method of single-strand conformation polymorphism analysis. Analysis of total RNA from a group of eight human skin fibroblast cell lines did not detect any sequence variations in the decorin cDNA. In contrast, the analysis detected three sequence(More)
Pelleting inoculated seed of Vigna sinensis, Glycine wightii cv. Clarence, Lupinus albus, Desmodium uncinatum cv. Silverleaf and Glycine max with neutral rock phosphate rather than lime did not improve the survival of their non-acid producing rhizobia. The rhizobia survived equally well when applied in a 15% w/v gum arabic slurry and not pelleted.(More)