The tumour suppressor gene p53 has been found to be mutated or inactivated at high frequency in several common human tumours. We have examined a series of exocrine pancreatic carcinomas for over-expression of mutant forms of p53 by immunohistochemistry with a panel of specific antibodies. We found immunodetectable p53 in 13 of 22 (60%) frozen pancreatic… (More)
OBJECTIVES To measure levels of anxiety, depression and post-traumatic stress among survivors of a critical illness and to relate these symptoms to general health parameters. DESIGN A prospective study of patients who had spent a minimum 3 days (median 9 days) in a general intensive care unit (ICU). Of these patients, 51 were interviewed 3 months after… (More)
Decreased membrane rigidity is one of the characteristics of malignant cells, resulting in part from the desaturation of stearic acid into oleic acid. In this study we investigated the influence of stearic acid on tumour cell inhibition in vitro and tumour development in vivo. Stearic acid inhibited the colony-forming ability of 4 out of 5 rat and two human… (More)
Writing a good review article is a real challenge. It requires not just a detailed literature search but a thorough 'digest' of the material obtained. Readers seek an up-to-date guide through a morass of data that has been sifted by the author and then integrated into a coherent and authoritative account.
Our previous phase II study of cisplatin and gemcitabine in malignant mesothelioma showed a 47.6% (95% CI 26.2-69.0%) response rate with symptom improvement in responding patients. Here we confirm these findings in a multicentre setting, and assess the effect of this treatment on quality of life and pulmonary function. Fifty-three patients with pleural… (More)
Carcinoma of the pancreas is the fourth leading cause of cancer death in the western world and has an overall 5-year survival of one per cent (Malagelada, 1979). During the past four decades the incidence trebled in the United States and Morgan & Wirmsley, 1977). One cause for the poor prognosis is the late diagnosis of the disease; the pancreas is deeply… (More)
Peripheral blood lymphocytes (PBL) were obtained from 13 patients and tumour-intrinsic lymphocytes (TIL) from 20 patients with colorectal cancer. The PBL were separated on a Ficoll-Isopaque gradient and the TIL by digestion of the tumour with collagenase-DNase. Both PBL and TIL were passed through nylon-wool columns and the eluted cells were co-cultured for… (More)