Roberto Pagliarini

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The Intravenous Glucose Tolerance Test is an experimental procedure used to study the glucose-insulin endocrine regulatory system. An open problem is to construct a model representing simultaneously the entire regulative mechanism. In the past three decades, several models have appeared, but they have not escaped criticisms and drawbacks. In this paper, the(More)
The study of efficient methods to deduce fluxes of biological reactions, by starting from experimental data, is necessary to understand the dynamics of a metabolic model, but it is also a central issue in systems biology. In this paper we report some partial results and related open problems regarding the efficient computation of regulation fluxes in(More)
A workflow for data analysis is introduced to synthesize flux regulation maps of a metabolic system from time series of data observed in laboratory. The procedure is successfully tested on a significant case study, the photosynthetic phenomenon called NPQ, which determines plant accommodation to environmental light. A previously introduced MP model of such(More)
Primary hyperoxaluria type I (PH1) is an autosomal-recessive inborn error of liver metabolism caused by alanine:glyoxylate aminotransferase (AGT) deficiency. In silico modeling of liver metabolism in PH1 recapitulated accumulation of known biomarkers as well as alteration of histidine and histamine levels, which we confirmed in vitro, in vivo, and in PH1(More)
Several human diseases are caused by metabolism defects. Discovering the mechanisms that govern the onset and progression of human metabolism-related diseases is not a straightforward process. Computational approaches, such as the flux balance analysis, have been successfully used to extract useful knowledge on the metabolic dysregulation processes from(More)
Inborn errors of metabolism (IEM) are genetic diseases caused by mutations in enzymes or transporters affecting specific metabolic reactions that cause a block in the physiological metabolic fluxes. Therapeutic treatment can be achieved either by decreasing the metabolic flux upstream of the block or by increasing the flux downstream of the block. The(More)
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