Robert Steinbauer

Philipp Milkereit3
Herbert Tschochner3
Joachim Griesenbeck3
3Philipp Milkereit
3Herbert Tschochner
3Joachim Griesenbeck
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Ribosome biogenesis is tightly linked to cellular growth. A crucial step in the regulation of ribosomal RNA (rRNA) gene transcription is the formation of the complex between RNA polymerase I (Pol I) and the Pol I-dependent transcription factor Rrn3p. We found that TOR inactivation leads to proteasome-dependent degradation of Rrn3p and a strong reduction in(More)
All nuclear RNA polymerases are phosphoprotein complexes. Yeast RNA polymerase I (Pol I) contains approximately 15 phosphate groups, distributed to 5 of the 14 subunits. Information about the function of the single phosphosites and their position in the primary, secondary and tertiary structure is lacking. We used a rapid and efficient way to purify yeast(More)
Eukaryotic RNA polymerase I (Pol I) is specialized in rRNA gene transcription synthesizing up to 60% of cellular RNA. High level rRNA production relies on efficient binding of initiation factors to the rRNA gene promoter and recruitment of Pol I complexes containing initiation factor Rrn3. Here, we determine the cryo-EM structure of the Pol I-Rrn3 complex(More)
Subject: Message from the Chair [This is a slightly revised version of a message sent to OPSF Talk on April 29, 2010. Eds.] Dear members of SIAG OPSF: The distribution of OPSF-Talk has been transferred successfully from the NIST server to the SIAM server. As I announced in OP-SF NET 17.2 in March, one advantage for us is that SIAM will make sure the active(More)
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