Membrane currents and changes in the intracellular Ca2+ concentration ([Ca2+]i) were measured in HEK293 cells transfected with the human P2X3 receptor (HEK293-hP2X3). RT-PCR and immunocytochemistry indicated the additional presence of endogenous P2Y1 and to some extent P2Y4 receptors. P2 receptor agonists induced inward currents in HEK293-hP2X3 cells with… (More)
Transcriptional networks defining stemness in adult neural stem cells (NSCs) are largely unknown. We used the proximal cis-regulatory element (pCRE) of the retina-specific homeobox gene 2 (rx2) to address such a network. Lineage analysis in the fish retina identified rx2 as marker for multipotent NSCs. rx2-positive cells located in the peripheral ciliary… (More)
Enhancers have been described to evolve by permutation without changing function. This has posed the problem of how to predict enhancer elements that are hidden from alignment-based approaches due to the loss of co-linearity. Alignment-free algorithms have been proposed as one possible solution. However, this approach is hampered by several problems… (More)
[This corrects the article DOI: 10.1371/journal.pone.0141487.].
The session presents two approaches to dynamically generated content for use on the Web and in offline presentations. These techniques utilize software applications to perform production tasks that were previously tedious and time-consuming, freeing the designer to focus on the creative process. The first technique enables high-resolution graphics to be… (More)
The session presents a case study of a Web site specifically designed as a RIA. The structure of the Web site, iknowbetter.com, is based upon traditional online forums wherein a community of members shares ideas and opinions within moderated or un-moderated discussion areas. Members, however, can also utilize Web cams and microphones to add audio/video… (More)
To date, the most common forms of real-time messaging between individuals on the Internet have been text-based, including e-mail and instant messaging. Studies have suggested that Internet users overall spend more hours online with e-mail and instant messaging (IM) than with all other web browsing.<sup>1</sup> Macromedia's new technology opens up exciting… (More)