Robert P. Finch

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Chilling of shoot cultures from Oryza sativa L. cv. Taipei 309, to 4 °C leads to conditions of oxidative stress. Tissue H2O2 was observed to increase more than fourfold by 8 d of chilling, and levels of reduced glutathione, which normally rise in growing shoot cultures at 25 °C, were considerably repressed in chilled cultures. Whilst the activity of(More)
Embryogenic callus initiated from basal segments of micropropagated shoots of Oryza rufipogon were used to initiate cell suspension cultures. After approximately 3 months these cultures were capable of yielding large numbers of protoplasts which underwent sustained division in agarose-solidified medium at a frequency comparable to that observed with(More)
A method was developed for the in vitro clonal propagation of shoots from a range of wild rice and other grass species that have important genetic traits such as drought resistance and salinity tolerance. The axenic multiple shoot cultures, which were suitable for DNA and protein extraction or direct protoplast isolation, could be maintained without(More)
Restriction analysis of mitochondrial (mt) DNA from 3-month-old callus cultures of the cytoplasmic male sterile rice, V41A, which contains S2 or “wild abortive” cytoplasm, and its fertile maintainer, V41B, showed the same BamHI restriction profiles as mtDNA from the corresponding leaf material. Similarly, mtDNA of rice (var. Taipei 309) from leaves, a(More)
Flow cytometry was used to provide a rapid and accurate assessment of electroporation-induced uptake of macromolecules into plant protoplasts. Rice protoplasts were electroporated in the presence of fluorescein isothiocyanate-conjugated dextran (FITC-dextran). After washing, the protoplasts were resuspended in a solution containing propidium iodide which(More)
From January 2013, The UK College of Chiropractors joins the partnership between the Chiropractic and Osteopathic College of Australasia (COCA) and the European Academy of Chiropractic (EAC) to publish Chiropractic & Manual Therapies. This new partnership will enable the journal to grow and flourish and further improve access to high quality research(More)
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