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UNLABELLED Translational control of gene expression has emerged as a major mechanism that regulates many biological processes and shows dysregulation in human diseases including cancer. When studying differential translation, levels of both actively translating mRNAs and total cytosolic mRNAs are obtained where the latter is used to correct for a possible(More)
Outgrowth of neurites in culture is used for assessing neurotrophic activity. Neurite measurements have been performed very slowly using manual methods or more efficiently with interactive image analysis systems. In contrast, medium-throughput and noninteractive image analysis of neurite screens has not been well described. The authors report the(More)
BACKGROUND DNA microarrays are popular tools for measuring gene expression of biological samples. This ever increasing popularity is ensuring that a large number of microarray studies are conducted, many of which with data publicly available for mining by other investigators. Under most circumstances, validation of differential expression of genes is(More)
BACKGROUND DNA microarrays provide data for genome wide patterns of expression between observation classes. Microarray studies often have small samples sizes, however, due to cost constraints or specimen availability. This can lead to poor random error estimates and inaccurate statistical tests of differential expression. We compare the performance of the(More)
Two independent studies failed to find evidence consistent with Council, Kirsch, and Hafner (1986), who argued that the repeatedly observed correlations between Tellegen's (1981) Absorption Scale (TAS) and hypnosis measures were artifacts of testing context, and de Groot, Gwynn, and Spanos (1988), who claimed evidence for a Gender x Context moderator(More)
MOTIVATION High-throughput screening (HTS) is an early-stage process in drug discovery which allows thousands of chemical compounds to be tested in a single study. We report a method for correcting HTS data prior to the hit selection process (i.e. selection of active compounds). The proposed correction minimizes the impact of systematic errors which may(More)
The revised form of the Absorption Scale extracted from Tellegen's Multidimensional Personality Questionnaire (Tellegen, 1981; Tellegen & Atkinson, 1974) and the Short Imaginal Processes Inventory (Huba, Aneshensel, & Singer, 1981), a self-report questionnaire concerned with daydreaming activity, were administered to 2 samples of Ss (N = 479, N = 476), who(More)
In this article, we report two experiments in which various measures thought to be related to hypnotizability were analyzed by stepwise discriminant analysis techniques. Absorption (Tellegen, 1981, 1982; Tellegen & Atkinson, 1974) and preference for an imagic style of thinking (Isaacs, 1982) were robust predictors of hypnotizability; each variable accounted(More)
MOTIVATION Labeling techniques are being used increasingly to estimate relative protein abundances in quantitative proteomic studies. These techniques require the accurate measurement of correspondingly labeled peptide peak intensities to produce high-quality estimates of differential expression ratios. In mass spectrometers with counting detectors, the(More)
MOTIVATION High-throughput screening (HTS) plays a central role in modern drug discovery, allowing for testing of >100,000 compounds per screen. The aim of our work was to develop and implement methods for minimizing the impact of systematic error in the analysis of HTS data. To the best of our knowledge, two new data correction methods included in(More)