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We describe a simple, quantitative assay for any amplifiable DNA sequence that uses a video camera to monitor multiple polymerase chain reactions (PCRs) simultaneously over the course of thermocycling. The video camera detects the accumulation of double-stranded DNA (dsDNA) in each PCR using the increase in the fluorescence of ethidium bromide (EtBr) that(More)
The 5'----3' exonuclease activity of the thermostable enzyme Thermus aquaticus DNA polymerase may be employed in a polymerase chain reaction product detection system to generate a specific detectable signal concomitantly with amplification. An oligonucleotide probe, nonextendable at the 3' end, labeled at the 5' end, and designed to hybridize within the(More)
The so-called " Great Firewall of China " operates, in part, by inspecting TCP packets for keywords that are to be blocked. If the keyword is present, TCP reset packets (viz: with the RST flag set) are sent to both endpoints of the connection, which then close. However, because the original packets are passed through the firewall unscathed, if the endpoints(More)
Capsicum is a lightweight operating system capability and sandbox framework planned for inclusion in FreeBSD 9. Capsicum extends, rather than replaces, UNIX APIs, providing new kernel primitives (sandboxed capability mode and capabilities) and a userspace sandbox API. These tools support the compartmentalization of monolithic UNIX applications into logical(More)
The major part of the DNA from polyoma virus has been shown to consist of circular base-paired duplex molecules without chain ends.'-' The intertwined circular form accounts for the ease of renaturation4 of this DNA and the failure of the strands to separate in strand-separating solvents. I-' In previous studiesl-3 a minor component, II, observed in(More)
In this paper we explore the tradeoffs between security and performance in anonymity networks such as Tor. Using probability of path compromise as a measure of security, we explore the behaviour of various path selection algorithms with a Tor path simulator. We demonstrate that assumptions about the relative expense of IP addresses and cheapness of(More)
QJUMP is a simple and immediately deployable approach to controlling network interference in datacenter networks. Network interference occurs when congestion from throughput-intensive applications causes queueing that delays traffic from latency-sensitive applications. To mitigate network interference, QJUMP applies Inter-net QoS-inspired techniques to(More)
Kinetically monitored, reverse transcriptase-initiated PCR (kinetic RT-PCR, kRT-PCR) is a novel application of kinetic PCR for high throughput transcript quantitation in total cellular RNA. The assay offers the simplicity and flexibility of an enzyme assay with distinct advantages over DNA microarray hybridization and SAGE technologies for certain(More)
Contemporary CPU architectures conflate virtualization and protection , imposing virtualization-related performance, programma-bility, and debuggability penalties on software requiring fine-grained protection. First observed in micro-kernel research, these problems are increasingly apparent in recent attempts to mitigate software vulnerabilities through(More)