Robert M Harding

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Banana bunchy top virus (BBTV) genomic ssDNA is capable of complementary strand synthesis in vitro without the addition of exogenous primers. We have demonstrated that the self-priming of BBTV can be attributed to a population of endogenous primers which are bound to the genomic DNA within the virions. The primer molecules appeared to be composed entirely(More)
Promoter regions associated with each of the six ssDNA components of banana bunchy top virus (BBTV) have been characterized. DNA segments incorporating the intergenic regions of BBTV DNA-1 to -6 were isolated and fused to the uidA (beta-glucuronidase) reporter gene to assess promoter activity. In tobacco cell suspensions, the BBTV DNA-2 and -6 promoters(More)
Banana bunchy top nanovirus has a multicomponent, circular single-stranded DNA genome comprising at least six integral components, BBTV DNA-1 to -6, which have been consistently associated with bunchy top disease worldwide. At least three other components, BBTV S1, S2 and Y, which have been isolated from Taiwanese BBTV isolates, do not appear to be integral(More)
Recommended citation: Footnote 1: " Shark " means any of the migratory species, subspecies or populations in the Class Chondrichthyes (which includes sharks, rays, skates and chimaeras) except the cover photograph, may be reproduced in whole or in part and in any form for educational and other non-profit purposes without special permission from the(More)