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We have characterized a flat cellular variant of HTLV-1 Tax-transformed rat fibroblasts, 5R, which is unresponsive to all tested NF-kappaB activating stimuli, and we report here its genetic complementation. The recovered full-length cDNA encodes a 48 kDa protein, NEMO (NF-kappaB Essential MOdulator), which contains a putative leucine zipper motif. This(More)
a cDNA library transfer system based on retroviral vectors has been developed for expression cloning in mammalian cells. The use of retroviral vectors results in stable cDNA transfer efficiencies which are at least 100-fold higher than those achieved by transfection and therefore enables the transfer and functional screening of very large libraries. In our(More)
active NF-␬B is released and translocated to the nucleus as a result of the proteolytic degradation of I␬B proteins. Phosphorylation of I␬B␣ on Ser-32 and Ser-36 targets the molecule for degradation by the ubiquitin-26S pro-teasome pathway. While the processes leading to the degradation of the I␬B proteins are relatively well under-de l'Expression Gé nique(More)
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