Robert J. Griffitt

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Metallic nanoparticles are among the most widely used types of engineered nanomaterials; however, little is known about their environmental fate and effects. To assess potential environmental effects of engineered nanometals, it is important to determine which species are sensitive to adverse effects of various nanomaterials. In the present study,(More)
Increasing use of metallic nanomaterials is likely to result in release of these particles into aqueous environments; however, it is unclear if these materials present a hazard to aquatic organisms. Because some dissolution of metal particles will occur, it is important to distinguish effects of nanoparticulates from dissolved metals. To address this issue,(More)
Research has demonstrated that metallic nanoparticles produce toxicity in aquatic organisms that is due largely to effects of particulates as opposed to release of dissolved ions. The present research examined the interplay of nanoparticle composition and dissolution on response of the zebrafish gill following exposure to toxic (nanocopper or nanosilver) or(More)
A novel custom microarray for largemouth bass (Micropterus salmoides) was designed with sequences obtained from a normalized cDNA library using the 454 Life Sciences GS-20 pyrosequencer. This approach yielded in excess of 58 million bases of high-quality sequence. The sequence information was combined with 2,616 reads obtained by traditional suppressive(More)
Increasing utilization of metallic nanomaterials in recent years implies an increasing rate of release to the environment, with potentially serious adverse effects on environmentally important species. Previously, we demonstrated that exposure to nanoparticulate silver for 24-48 h results in dramatic alterations in global gene expression patterns and(More)
The use of nanoparticulate silver (AgNP) is increasingly widespread and recently has been shown to have a plausible release route into aquatic environments. To date, relatively little research has examined the effects of AgNP on estuarine fish. The authors present data indicating that chronic exposure to low levels of AgNP induces significant adverse(More)
The common molecular biology techniques, suppressive-subtractive hybridization (SSH) and semi-quantitative real-time PCR (SQRT-PCR), were used to identify differentially expressed genes in the grass shrimp, Palaemonetes pugio following exposure to three different xenobiotics. Lab-acclimated adult male grass shrimp were exposed to empirically derived 96-hr(More)
Nanomaterials are a diverse group of compounds whose inevitable release into the environment warrants study of the fundamental processes that govern the ingestion, uptake and accumulation in aquatic organisms. Nanomaterials have the ability to transfer to higher trophic levels in aquatic ecosystems, and recent evidence suggests that the surface chemistry of(More)
BACKGROUND Queen conch (Strombus gigas) reproduction is inhibited in nearshore areas of the Florida Keys, relative to the offshore environment where conchs reproduce successfully. Nearshore reproductive failure is possibly a result of exposure to environmental factors, including heavy metals, which are likely to accumulate close to shore. Metals such as Cu(More)
Serial analysis of gene expression (SAGE) is a transcriptome-profiling technique that allows accurate gene expression profiles to be generated without any need for prior sequence knowledge, making it ideal for assaying toxicant-specific genetic responses in nonmodel species. We used SAGE to perform a large-scale survey of the Palaemonetes pugio(More)