Robert J. Grabowski

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The generation of transport vesicles at the endoplasmic reticulum (ER) depends on cytosolic proteins, which, in the form of subcomplexes (Sec23p/Sec24p; Sec13p/Sec31p) are recruited to the ER membrane by GTP-bound Sar1p and form the coat protein complex II (COPII). Using affinity chromatography and two-hybrid analyses, we found that the essential COPII(More)
By complementation of a salt-sensitive mutant of Saccharomyces cerevisiae, we cloned the SOP1 gene, encoding a 114.5-kDa protein of 1033 amino acids. Cells deleted for SOP1 exhibited sensitivity to sodium stress, but showed no sensitivity to general osmotic stress. Following exposure of sop1Delta cells to NaCl stress, the intracellular Na+ level and the(More)
A clone designated A.t.RAB6 encoding a small GTP-binding protein was isolated from a cDNA library of Arabidopsis thaliana leaf tissue. The predicted amino acid sequence was highly homologous to the mammalian and yeast counterparts, H.Rab6 and Ryh1/Ypt6, respectively. Lesser homology was found between the predicted Arabidopsis protein sequence and two small(More)
Docking of ER-derived vesicles to the cis-Golgi compartment in yeast requires vesicle and target membrane receptors (v-SNAREs and t-SNAREs) and the GTPase Ypt1p. The t-SNARE Sed5p is complexed with Sly1p in vivo. The mutant form Sly1-20p rescues Ypt1p-lacking cells from lethality, suggesting an inhibitory function of Sly1p in v-SNARE/t-SNARE interaction.(More)
In this paper, we describe the design and development of a network of large unmanned ground vehicles (LUGVs) using low-cost, off-the-shelf technology and building upon experience gained at the 2005 Defense Advanced Research Projects Agency (DARPA) Grand Challenge. Our unique distributed architecture allows robots with different capabilities to interact,(More)
Ras-related, guanine nucleotide-binding proteins of the Ypt/Rab family play a key role at defined steps in vesicular transport, both in yeast and in mammalian cells. In yeast, Ypt1p has an essential function late in endoplasmic reticulum (ER) to Golgi transport, and the redundant Ypt31/Ypt32 GTPases have been proposed to act in transport through and/or from(More)
The MITRE Meteor team fielded an autonomous vehicle that competed in DARPA’s 2005 Grand Challenge race. This paper describes the team’s approach to building its robotic vehicle, the vehicle and components that let the vehicle see and act, and the computer software that made the vehicle autonomous. It presents how the team prepared for the race and how their(More)
The Defense Advanced Research Projects Agency (DARPA) challenged autonomous ground vehicle developers in the "2005 DARPA Grand Challenge" to build a vehicle that could complete a 132 mile course through the American desert southwest. MITRE, a not-for-profit systems engineering company, responded to this challenge by creating the MITRE Meteor in just 11(More)
This paper presents an on-line algorithm which provides high signal to noise ratio heading predictions for unmanned ground vehicles. The algorithm uses cross correlation of SICK laser scans to improve the heading predictions from GPS. It is tested on our ground vehicles in outdoor urban environment and verified to provide accurate smooth heading predictions(More)
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